贵州汉族人群载脂蛋白B基因EcoRI位点多态性与原发性高血压相关性的研究

发布时间：2018-05-21 06:54

本文选题：原发性高血压 + 载脂蛋白B　；参考：《贵州医科大学》2017年硕士论文

【摘要】：目的:研究贵州地区汉族人群载脂蛋白B(apoB)基因EcoRI位点多态性与原发性高血压(EH)的关系,并探讨该位点的多态性与血脂及其他心血管危险因素的关系。方法:采用聚合酶链反应-限制性片段长度多态性的方法分别检测81例高血压患者及71例血压正常者的apoB基因EcoRI位点的多态性,比较该位点不同基因型的频率、等位基因的频率,并对血脂、体重指数(BMI)、肌酐、尿素氮、糖化血红蛋白(HbAlc)等指标进行测定。结果:(1)在正常对照组中,apoB基因EcoRI位点的E+E+和E+E-基因型频率分别为0.887和0.113;在高血压组中,EcoRI位点的E+E+、E+E-基因型频率分别为0.864和0.136;两组均未检测到E-E-基因型;经比较,未发现E+E+和E+E-基因型频率在两组间的差异有统计学意义(P0.05)。(2)正常组中E+和E-等位基因的频率分别为0.944和0.056;高血压组中E+和E-等位基因的频率分别为0.932和0.068;经比较,两组在该位点的等位基因频率的差异均无统计学意义(P0.05)。(3)在EH组中,携带E+E-基因型个体的HbAlc和低密度脂蛋白胆固醇(LDL-C)水平显著高于携带E+E+基因型个体,而BMI、肌酐、尿素氮、甘油三酯(TG)、总胆固醇(TC)、高密度脂蛋白胆固醇(HDL-C)、LDL-C/HDL-C比值、TC/HDL-C比值以及脂蛋白a[LP(a)]等在两组中EcoRI位点基因型频率的差异均无统计学意义(P0.05);在正常对照组中,上述指标与EcoRI位点的多态性无明显的相关性(P0.05)。结论:本研究发现apoB基因EcoRI位点多态性与贵州汉族人群原发性高血压的发生无关,但该位点的多态性与高血压患者HbAlc和LDL-C水平有关。
[Abstract]:Objective: to study the relationship between EcoRI polymorphism of apolipoprotein (apoB) gene and essential hypertension (EH) in Guizhou Han population, and to explore the relationship between polymorphism of ApoB gene and blood lipids and other cardiovascular risk factors. Methods: polymerase chain reaction-restriction fragment length polymorphism (PCR) was used to detect the polymorphism of apoB gene EcoRI locus in 81 patients with hypertension and 71 patients with normal blood pressure, and the frequencies of different genotypes of this locus were compared. The frequency of alleles and serum lipids, body mass index (BMI), creatinine, urea nitrogen, HbAlc were measured. Results the frequencies of E E and E E-genotype at EcoRI site of apoB gene were 0.887 and 0.113 in normal control group, 0.864 and 0.136 in hypertensive group, respectively. The frequencies of E and E- alleles in normal controls were 0.944 and 0.056 respectively, and the frequencies of E and E- alleles in hypertension group were 0.932 and 0.068, respectively. In EH group, the levels of HbAlc and LDL-C in E- genotype individuals were significantly higher than those in E E genotype individuals, while BMI, creatinine, urea nitrogen were significantly higher in EH group. There was no significant difference in the genotype frequency of EcoRI locus between the two groups, such as triglyceride, total cholesterol, HDL-C / HDL-C ratio and lipoprotein a, in the two groups; in the normal control group, there was no significant difference in the genotypic frequencies of EcoRI locus between the two groups (P 0.05), but in the normal control group, there was no significant difference in the genotype frequency of EcoRI locus between the two groups. There was no significant correlation between the above indexes and the polymorphism of EcoRI locus (P 0.05). Conclusion: this study found that the polymorphism of EcoRI locus of apoB gene was not related to the occurrence of essential hypertension in Guizhou Han population, but the polymorphism of this locus was related to the levels of HbAlc and LDL-C in patients with hypertension.
【学位授予单位】：贵州医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R544.11

【参考文献】