维生素D受体基因多态性与环境因素交互作用对河南省结核病发病影响

发布时间：2018-05-21 07:02

本文选题：结核病 + 维生素D受体　；参考：《现代预防医学》2017年11期

【摘要】：目的为揭示环境因素及维生素D受体(VDR)基因SNP rs2222570单核苷酸多态性与河南地区结核病发病相关性。方法本研究采样病例对照研究设计,于2016年2月至10期间在郑州市结防所收集涂阳肺结核病人140例,并以年龄性别匹配取同时期140例健康体检人群作为对照。对抽取人群进行问卷调查和静脉血采集,并用聚合酶链反应结合测序结果分析对维生素D受体基因SNP rs2222570多态性。进行单因素卡方检验和多因素非条件Logistics回归分析。结果多因素logistics回归表明结核病接触史(OR=21.38 95%CI=6.4~36.08),吸烟(OR=4.27 95%CI=2.34~9.52)、粉尘接触史(OR=2.84 95%CI=1.95~6.97)、卡介苗接种(OR=0.47 95%CI=0.007~0.33)、睡眠时间(OR=0.14 95%CI=0.02~0.76)和维生素D受体基因SNP rs2222570基因型C/C(OR=0.72 95%CI=0.55~0.93)与结核发病相关性有统计学意义。VDR基因C/C基因型作为遗传因素与环境因素中卡介苗接种、吸烟和结核病人接触史均存在交互作用。结论 VDR基因SNP rs2222570多态性与传统环境因素在结核病发生中存在一定交互作用。该研究结果为了解结核病发生、发展机制,预防控制该病的发生和流行提供了科学依据。
[Abstract]:Objective to investigate the association between environmental factors and SNP rs2222570 single nucleotide polymorphism of vitamin D receptor (VDR) gene in Henan province. Methods from February to October 2016, 140 smear positive pulmonary tuberculosis patients were collected from the Jiefang Institute of Zhengzhou from February to October 2016, and 140 healthy people matched by age and sex were used as control. The SNP rs2222570 polymorphism of vitamin D receptor gene was analyzed by polymerase chain reaction (PCR) and sequencing. Single factor chi-square test and multivariate non-conditional Logistics regression analysis were performed. Results the multivariate logistics regression analysis showed that the history of tuberculosis exposure was 21.38 95CII 6.46.08, smoking 4.27 95CI2.34 / 9.52, dust exposure 2.84,95CI1.95CI1.956.97, BCG 0.47 95CI0.0070.33, sleep time 0.14 95CI0.020.76) and vitamin D receptor gene SNP rs2222570 genotype C/C(OR=0.72 95CI0.55c0.93). The C / C genotype of VDR gene was inoculated with BCG as a genetic and environmental factor. There was an interaction between smoking and history of exposure to tuberculosis. Conclusion there is some interaction between VDR gene SNP rs2222570 polymorphism and traditional environmental factors in the occurrence of tuberculosis. The results provide scientific basis for understanding the occurrence and development mechanism of tuberculosis and preventing and controlling the occurrence and prevalence of the disease.
【作者单位】：河南省疾病预防控制中心;
【分类号】：R181.3;R52

【相似文献】