尼罗罗非鱼γ-干扰素基因的克

发布时间：2018-05-21 13:24

本文选题：尼罗罗非鱼 + γ-干扰素　；参考：《南方水产科学》2017年01期

【摘要】：γ-干扰素(interferon,IFN)是调控天然免疫与细胞免疫的一种重要细胞因子,在抵抗病毒入侵和细菌感染中发挥重要作用。该研究从尼罗罗非鱼(Oreochromis niloticus)脾脏组织中扩增到γ-干扰素基因的c DNA,大小为621 bp,编码206个氨基酸。生物信息学分析表明,尼罗罗非鱼γ-干扰素分子(On IFNγ)具有信号肽、IFN特征序列及核定位信号。预测的三级结构与人类IFNγ晶体结构最相似。q PCR检测显示,IFNγ在正常鱼体内的脾脏中表达量最高,其次是肠、鳃和肾脏。感染无乳链球菌(Streptococcus agalactiae)后,On IFNγ在脾脏、肾和鳃中的表达均显著上调(P0.05)。On IFNγ在相关免疫器官中的高表达、受感染后的表达上调,说明其在罗非鱼免疫防御中可能具有重要作用。该研究为进一步开展On IFNγ基因的功能研究、探讨其在抗病转基因及抗病选育中的开发应用奠定了基础。
[Abstract]:Interferon- 纬 (IFN) is an important cytokine regulating innate and cellular immunity, which plays an important role in resisting virus invasion and bacterial infection. In this study, the cDNA of interferon 纬 gene was amplified from the spleen of Oreochromis niloticus. The cDNA of interferon 纬 gene was 621 BP, encoding 206 amino acids. Bioinformatics analysis showed that the nilotilapia interferon 纬 (on IFN 纬) has the characteristic sequence of signal peptide IFN and nuclear localization signal. The predicted tertiary structure was the most similar to that of human IFN 纬 crystal structure. The detection of PCR showed that the expression of IFN- 纬 was the highest in the spleen of normal fish, followed by intestine, Gill and kidney. After infection with Streptococcus agalactiae, the expression of on IFN 纬 in spleen, kidney and Gill was upregulated significantly, and the expression of on IFN 纬 in related immune organs was upregulated, which suggested that it might play an important role in the immune defense of tilapia. This study laid a foundation for further research on the function of on IFN 纬 gene and its development and application in transgenic and breeding of disease resistance.
【作者单位】：中国水产科学研究院珠江水产研究所农业部热带亚热带水产资源利用与养殖重点实验室;上海海洋大学水产与生命学院;
【基金】：国家自然科学基金项目(31272688) 国家高技术研究发展计划(863计划)项目(2011AA100404) 现代农业产业技术体系建设专项资金(CARS-49)
【分类号】：S917.4

【参考文献】