cry1Ac基因启动子表达Vip3Aa蛋白特性分析

发布时间：2018-05-22 11:38

  本文选题：苏云金芽胞杆菌 + cryAc启动子　； 参考：《东北农业大学学报》2017年09期

【摘要】：苏云金芽胞杆菌cry基因启动子常用于构建蛋白表达载体。为探讨苏云金芽胞杆菌cry基因启动子指导Vip3Aa蛋白表达情况及杀虫活性,以p UC19载体为基础,运用重叠引物PCR方法构建Vip3Aa11表达载体,并与由T7启动子指导的Vip3Aa11表达蛋白杀虫活性、抗胰蛋白酶稳定性比较,初步探索发酵条件。结果表明,cry1Ac启动子与T7启动子均在上清液中表达大小为88 ku Vip3Aa11蛋白,对甜菜夜蛾、棉铃虫杀虫活性差异不显著,cry1Ac基因启动子在37℃、48 h更适合Vip3Aa11蛋白的表达,为vip基因表达、功能验证及杀虫机理等研究提供新思路。
[Abstract]:The promoter of cry gene of Bacillus thuringiensis is often used to construct protein expression vector. In order to investigate the expression and insecticidal activity of cry gene promoter of Bacillus thuringiensis, based on p UC19 vector, the Vip3Aa11 expression vector was constructed by using overlapping primer PCR method, and the insecticidal activity of Vip3Aa11 expression protein guided by T7 promoter was obtained. The stability of antitrypsin was compared and the fermentation conditions were preliminarily explored. The results showed that both cry1Ac promoter and T7 promoter expressed 88 ku Vip3Aa11 protein in supernatant. The difference of insecticidal activity of Helicoverpa armigera was not significant. Cry1Ac promoter was more suitable for the expression of Vip3Aa11 protein at 37 鈩，

本文编号：1921905