MIF基因rs755622位点多态性与广西壮族人群炎症性肠病的关系

发布时间：2018-05-22 15:23

本文选题：炎症性肠病 + 溃疡性结肠炎　；参考：《山东医药》2017年08期

【摘要】：目的探讨巨噬细胞移动抑制因子(MIF)基因rs755622位点多态性与广西壮族人群炎症性肠病(IBD)的关系。方法选择广西壮族溃疡性结肠炎(UC)患者144例(UC组)、克罗恩病(CD)患者101例(CD组),另选广西壮族健康志愿者202例(对照组),取各组肠黏膜组织,抽提基因组DNA,采用PCR技术扩增MIF基因目的片段,并进行产物测序,结果在Gen Bank数据库上进行序列比对。结果所有目的片段的测序结果与Gen Bank数据库上序列一致。以CC基因型为参照,UC组GG、GC基因型与对照组同基因型比较差异均无统计学意义(P均0.05)。以CC基因型为参照,CD组GG、GC基因型与对照组同基因型比较差异均无统计学意义(P均0.05)。UC组、CD组G、C等位基因频率与对照组比较差异均无统计学意义(P均0.05)。结论 MIF基因rs755622位点多态性与广西壮族人群IBD的易感性无明显相关性。
[Abstract]:Objective to investigate the relationship between rs755622 polymorphism of macrophage migration inhibitory factor (MIF) gene and inflammatory bowel disease (IBD) in Zhuang nationality in Guangxi. Methods 144 patients with ulcerative colitis and 101 patients with Crohn's disease and 202 healthy volunteers from Guangxi Zhuang nationality were selected. Genomic DNA was extracted, MIF gene fragment was amplified by PCR technique, and the product was sequenced. The results were compared with Gen Bank database. Results the sequence of all target fragments was consistent with that of Gen Bank database. Compared with the control group, there was no significant difference between the GGG GC genotype and the control group in CC genotype (P < 0.05). There was no significant difference in GGG C allele frequency between 0.05).UC group and control group compared with control group (P < 0. 05), and there was no significant difference in allele frequency between CD group and control group (P < 0. 05). Conclusion the polymorphism of rs755622 locus of MIF gene is not associated with the susceptibility to IBD of Zhuang nationality in Guangxi.
【作者单位】：广西医科大学第一附属医院;
【基金】：国家自然科学基金资助项目(81460114) 广西医科大学青年科学基金(GXMUYSF201334)
【分类号】：R574.62

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