11株H3N2亚型禽流感病毒广西分离株全基因序列测定与分析

发布时间：2018-05-23 07:44

本文选题：禽流感病毒 + HN亚型　；参考：《中国兽医学报》2017年05期

【摘要】：2013—2014年从广西活禽市场采集的棉拭子样品中分离鉴定出11株H3N2亚型禽流感病毒(AIV)。为了解该亚型AIV的分子特征及其遗传进化关系,本研究对分离株进行全基因序列测定并采用生物学信息软件对其进行分析。结果显示,11株H3N2亚型AIV HA基因裂解位点均只有1个碱性氨基酸,符合低致病性禽流感的分子特征;HA基因受体结合位点为242Q~244G,不同于人流感226L~228S,优先与禽源受体进行结合;全基因遗传进化分析表明11株H3N2亚型AIV分离株的8个基因片段均属于欧亚谱系的禽源进化分支,与猪源和人源分离株的亲缘关系较远;分离株内部基因来源较复杂,推测这11株毒株是由不同亚型毒株经过长时间进化而发生自然重排形成的重组病毒。
[Abstract]:From 2013 to 2014, 11 H3N2 subtypes of avian influenza virus (AIV) were isolated and identified from the cotton swab samples collected from the live poultry market in Guangxi. In order to understand the molecular characteristics and genetic evolution of the subtype AIV, the total gene sequencing of the isolates and the biological information soft pieces were used to analyze the isolates. The results showed that 11 H3N2 subtypes were found. The AIV HA gene cleavage site has only 1 basic amino acids, which conforms to the molecular characteristics of the low pathogenic avian influenza. The binding site of the HA gene receptor is 242Q~244G, unlike human influenza 226L~228S, and is preferred to combine with the avian source receptor. The whole gene genetic evolution analysis shows that the 8 gene fragments of the 11 H3N2 subtype AIV isolates belong to the Eurasian lineage. The fowl evolution branch is far from the relationship between the pig source and the human source, and the internal gene source of the isolates is more complex. The 11 strains of the isolates are the recombinant viruses which are naturally rearranged by different subtypes of virulates after a long time evolution.
【作者单位】：广西师范大学生命科学学院;广西壮族自治区兽医研究所广西畜禽疫苗新技术重点实验室;
【基金】：广西特聘专家专项基金资助项目(2011B020) 广西科技攻关重大专项基金资助项目(1222003-2-4,14121003-4-2)
【分类号】：S852.65

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