缺钾对不同基因型香蕉根系分泌物产生及土壤钾活化的影响

发布时间：2018-05-23 09:51

本文选题：香蕉 + 基因型　；参考：《西南农业学报》2017年03期

【摘要】：为了探讨低钾胁迫对香蕉幼苗生长和根系分泌物的影响,以及不同香蕉基因型对土壤钾活化差异。通过离位溶液培养法收集香蕉根分泌物,经低温冷冻干燥器浓缩后,进行土壤钾活化试验,利用高效液相色谱(HPLC)测定分泌物中有机酸成分及含量。结果表明,在低钾胁迫条件下,钾高效基因型香蕉幼苗鲜质量显著高于钾低效基因型。香蕉根系分泌物中的有机酸色谱图比较简单,钾低效基因型品种仅发现3种有机酸:草酸、酒石酸和柠檬酸,钾高效基因型品种发现了4种有机酸:是草酸、酒石酸、柠檬酸和琥珀酸。钾高效基因型根系有机酸、蛋白质和可溶性糖的分泌能力显著高于低效基因型,且含量变化与鲜质量变化趋势一致,总酚酸变化趋势正好相反。不同基因型香蕉根系分泌物对土壤钾素均有活化作用,其中广粉活化能力最强,各基因型间差异达极显著水平。低钾胁迫条件下,香蕉分泌大量有机物质,基因型之间差异较为明显,香蕉钾高效基因型根系有机酸的分泌能力和土壤钾的活化能力远高于低效基因型。
[Abstract]:In order to study the effects of low potassium stress on the growth and root exudates of banana seedlings and the difference of potassium activation between different banana genotypes. Banana root exudates were collected by culture in vitro and concentrated in a cryopreservation dryer. The soil potassium activation test was carried out. The organic acids and their contents in the secretions were determined by high performance liquid chromatography (HPLC). The results showed that the fresh quality of banana seedlings with high potassium efficiency was significantly higher than that with low potassium stress. The chromatogram of organic acids in banana root exudates was relatively simple. Only three organic acids were found in potassium inefficient genotypes: oxalic acid, tartaric acid and citric acid, and four organic acids were found in potassium efficient genotypes: oxalic acid and tartaric acid. Citric acid and succinic acid. The secretion ability of organic acid, protein and soluble sugar in root of potassium efficient genotype was significantly higher than that of low effective genotype, and the change of content was consistent with that of fresh quality, and the change trend of total phenolic acid was opposite. The root exudates of different genotypes could activate potassium in soil, and the activation ability of broad powder was the strongest, and the difference between genotypes was very significant. Under low potassium stress, banana secreted a large amount of organic matter, and the difference between genotypes was obvious. The ability of root organic acid secretion and the activation energy of soil potassium of banana potassium efficient genotypes were much higher than those of low-efficient genotypes.
【作者单位】：海南省农业科学院农业环境与土壤研究所/农业部海南耕地保育科学观测试验站;
【基金】：海南省自然科学基金项目(20153097) 海南省农业科学院农业科技创新专项(CXZX201420)
【分类号】：S668.1

【相似文献】