钙对染铅大鼠海马NMDAR和mGluR5受体介导相关基因mRNA及CaMKⅡ蛋白表达的影响

发布时间：2018-05-23 12:27

  本文选题：钙 + 铅　； 参考：《营养学报》2017年01期

【摘要】：目的通过研究钙对染铅大鼠海马NMDAR和m Glu R5受体介导相关基因mRNA及CaMKII蛋白表达的影响,为进一步以补钙预防铅的危害提供理论依据。方法选择体质量(50±2)g的清洁级雄性SD大鼠60只,共分五组,每组12只,分别为对照组(基础饲料,蒸馏水)、染铅组(基础饲料,0.2%醋酸铅饮水)、试验Ⅰ组(基础饲料添加0.5%碳酸钙,0.2%醋酸铅饮水)、试验Ⅱ组(基础饲料添加1%碳酸钙,0.2%醋酸铅饮水)和试验Ⅲ组(基础饲料添加2%碳酸钙,0.2%醋酸铅饮水)。连续饲养60 d后,取海马,用荧光定量RT-PCR法测定海马中Grm5、Camk2a、Grin1、Homer1、Prkca和ERK2的mRNA表达量,Western-blotting测定大鼠海马中CaMKII的蛋白表达量。结果与对照组相比,染铅组海马中Grm5、Camk2a、Grin1、Homer1、Prkca、ERK2的mRNA表达量及CaMKII的蛋白表达量均极显著降低(P0.01)。与染铅组相比,试验组钙浓度在一定范围内越高,目的基因表达量相对升高越多,试验Ⅱ组CaMKII的蛋白表达量极显著上升(P0.01)。结论一定浓度的钙可干预铅致大鼠海马组织的损伤。
[Abstract]:Objective to study the effect of calcium on the expression of NMDAR and m Glu R5 receptor mediated genes mRNA and CaMKII in hippocampus of lead exposed rats, and to provide theoretical basis for calcium supplementation to prevent the harm of lead. Methods Sixty male SD rats of clean grade with 50 卤2g body weight were divided into five groups, 12 rats in each group, as control group. Distilled water, lead group (basic feed 0.2% lead acetate drinking water), test I group (basic feed supplemented with 0.5% calcium carbonate 0.2wt% lead acetate drinking water), test 鈪，

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