焉耆马的DRA与DQA基因遗传多态性及其序列分析

发布时间：2018-05-23 15:12

本文选题：焉耆马 + 主要组织相容性复合体　；参考：《中国畜牧杂志》2017年05期

【摘要】：采用聚合酶链式反应-单链构象多态性(PCR-SSCP)方法检测焉耆马ELA-DRA*exon2、ELADQA*exon2的基因型,并对不同等位基因进行测序,对其核苷酸与氨基酸序列进行分析。结果表明:焉耆马的ELA-DRA*exon2共检出4个等位基因;ELA-DQA*exon2共检出9个等位基因,通过核苷酸序列比对发现其中3个为新等位基因。ELA-DRA*exon2、ELA-DQA*exon2的各等位基因序列分析结果表明,发生非同义替换的多态位点均主要分布在抗原结合位点对应的编码序列中。遗传多态性分析结果表明,焉耆马的ELA-DRA*exon2多态信息含量为0.4,属于中度多态。卡方检验结果表明,焉耆马在该ELA-DRA*exon2位点处于Hardy-Weinberg平衡(χ2χ20.05)。本研究为马的抗病育种提供了理论基础。
[Abstract]:Polymerase chain reaction-single strand conformation polymorphism (SSCP) was used to detect the genotype of ELA-DRAexon 2 and ELADQAX exon2 in Yanqi horse. Different alleles were sequenced and their nucleotide and amino acid sequences were analyzed. The results showed that four alleles (ELA-DQAexon2) were detected by ELA-DRA*exon2 in Yanqi and 9 alleles were detected by nucleotide sequence alignment. The results of alleles analysis showed that three of the alleles were new alleles. ELA-DRAexon2ELA-DQAexon2 alleles were found to be ELA-DQAexon2 alleles. The polymorphic sites with nonsynonymous substitutions are mainly distributed in the coding sequences corresponding to antigen-binding sites. The results of genetic polymorphism analysis showed that the ELA-DRA*exon2 polymorphism information content of Yanqi was 0.4, which belonged to moderate polymorphism. The results of chi-square test showed that Yanqi was in Hardy-Weinberg equilibrium at the ELA-DRA*exon2 site (蠂 2 蠂 20.05). This study provides a theoretical basis for horse breeding for disease resistance.
【作者单位】：新疆畜牧科学院畜牧研究所;
【基金】：新疆维吾尔自治区公益性科研院所基本科研业务经费资助项目(Ky Gy2016004) 新疆绒毛用羊遗传育种与繁殖重点实验室(XJYS1105)
【分类号】：S821

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