滋养叶细胞增殖基因及间质细胞凋亡检测在水泡状胎块诊断中的价值

发布时间：2018-05-24 17:58

本文选题：HM + Ki　；参考：《中国现代医学杂志》2017年22期

【摘要】：目的探讨滋养叶细胞增殖相关基因及间质细胞凋亡检测在水泡状胎块(HM)病理诊断中的价值。方法应用免疫组织化学标记技术,检测100例HM(其中完全性HM 61例,部分性HM 39例)及40例绒毛水肿的病理组织标本滋养叶细胞的Ki67、Livin与Bcl-2的表达,并应用TUNEL法对绒毛间质凋亡细胞进行原位观察。结果 Ki67阳性表达在绒毛水肿组、部分性HM组、完全性HM组趋势呈逐渐增强,各组间比较均差异有统计学意义(P0.05);绒毛水肿组及HM组绒毛间质内AI指数比较,差异有统计学意义(P0.05);Livin及Bcl-2表达差异无统计学意义(P0.05);Ki67表达与AI指数呈正相关(P0.05)。结论间质细胞凋亡联合滋养叶细胞中Ki67表达检测可用于HM的诊断与分型,也可用于鉴别HM与绒毛水肿。
[Abstract]:Objective to investigate the value of the detection of trophoblastic proliferation-associated genes and interstitial cell apoptosis in the pathological diagnosis of HM. Methods Immunohistochemistry was used to detect the expression of Ki67 livin and Bcl-2 in trophoblastic cells from 100 cases of HMs (including 61 cases of complete HM and 39 cases of partial HM) and 40 cases of chorionic edema. The apoptosis of villous stromal cells was observed in situ by TUNEL method. Results the positive expression of Ki67 in chorionic villous edema group, partial HM group and complete HM group was gradually increased, and there were significant differences among the three groups (P 0.05), and the AI index in chorionic villi edema group and HM group was higher than that in HM group. There was no significant difference in the expression of Livin and Bcl-2. There was a positive correlation between the expression of P0.05 and Ki67 and AI index (P 0.05). Conclusion the detection of Ki67 expression in stromal cell apoptosis combined with trophoblast can be used for the diagnosis and typing of HM and for differentiating HM from chorionic edema.
【作者单位】：上海市普陀区妇婴保健院病理科;上海市普陀区妇婴保健院妇科;
【基金】：上海市普陀区卫生系统自主创新科研资助项目(No:普KW2012308) 上海市普陀区临床重点专科建设项目(No:PW-2-14-02)
【分类号】：R737.33

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