OAS1基因多态性与EV71感染易感性的相关研究

发布时间：2018-05-24 21:16

本文选题：肠道病毒71型 + 脑炎　；参考：《青岛大学》2016年硕士论文

【摘要】：目的探讨肠道病毒71型(EV71)感染患儿OAS1基因rs1131454位点单核苷酸多态性与肠道病毒71型(EV71)感染易感性之间的关系,并探讨不同基因型对肠道病毒71型(EV71)感染病情严重程度的影响。方法选取2012年6月至2014年9月肠道病毒71型(EV71)感染的患儿216例及同时同地同年龄组健康儿童441例。序列特异性引物-聚合酶链反应(PCR-SSP)检测肠道病毒71型(EV71)感染患儿及对照组健康儿童OAS1基因rs1131454位点的单核苷酸多态性、收集其临床资料并进行外周血样本检测。结果1.在本研究中,216例EV71感染手足口病患儿,其中轻症126例,重症90例,其中41例表现为脑炎,1例死亡。所有患者入院时体温≥37℃。2.OAS1 rs1131454基因型及等位基因频率在EV71感染组及健康对照组之间没有存在显著差异(P0.05)。3.OAS1 rs1131454位点基因型及等位基因频率在EV71感染轻症组与重症组之间存在差异(χ2=8.498,P=0.014;χ2=8.598,P=0.003),但在重症组与脑炎组之间无明显差异(P0.05)。4.在肠道病毒71型感染组中OAS1 rs1131454位点在患者年龄、发热持续时间、血白细胞计数、血C反应蛋白水平、血糖浓度等方面,不同基因型之间差异显著。但在性别、外周血中丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)水平,在不同基因型之间的差异无统计学意义(P0.05)。5.41例脑炎患者中OAS1 rs1131454位点在脑脊液中葡萄糖浓度、氯化物浓度及单核细胞计数等方面,不同基因型之间无显著差异(P0.05)。结论1.OAS1 rs1131454位点基因多态性与EV71感染致病轻重程度之间有一定程度的相关性,且携带OAS1 rs1131454A等位基因的感染患儿进展为重症的概率较高。2.OAS1 rs1131454位点基因多态性与EV71感染的患病风险没有明显相关性。
[Abstract]:Objective to investigate the relationship between the single nucleotide polymorphism of OAS1 gene rs1131454 loci and the susceptibility to enterovirus 71 (EV71) infection in children with enterovirus 71 (EV71) infection, and to explore the effect of different genotypes on the severity of enterovirus 71 (EV71) infection. Methods from June 2012 to September 2014, the sense of type 71 (EV71) of enterovirus was selected. 216 children infected and 441 healthy children with same age group. Sequence specific primer polymerase chain reaction (PCR-SSP) was used to detect the single nucleotide polymorphisms of the OAS1 gene locus of the enterovirus 71 (EV71) infected children and the control group of healthy children. The clinical data were collected and the peripheral blood samples were tested. Results 1. were in the study. In the study, 216 cases of EV71 infected hand foot and mouth disease, of which 126 cases were light disease and 90 cases were severe, of which 41 cases were encephalitis and 1 died. There was no significant difference (P0.05).3.OAS1 rs1131454 loci genotype between the EV71 infection group and the healthy control group at the temperature of more than 37 centigrade and the allele frequency of all patients at admission. The allele frequency was different between the EV71 infection group and the severe group (x 2=8.498, P=0.014, X 2=8.598, P=0.003), but there was no significant difference between the severe group and the encephalitis group (P0.05).4. in the enterovirus 71 infection group, the OAS1 rs1131454 loci was in the patient's age, the duration of fever, the blood leukocyte count, the blood C reactive protein level, and the blood There were significant differences among different genotypes, but in sex, the levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in the peripheral blood were not significant between different genotypes (P0.05), the concentration of glucose, chloride concentration and monocyte in the cerebrospinal fluid of OAS1 rs1131454 sites in.5.41 patients with encephalitis There was no significant difference between different genotypes (P0.05). Conclusion there is a correlation between the polymorphism of the 1.OAS1 rs1131454 locus and the severity of EV71 infection, and the progression of the infected children with OAS1 rs1131454A allele is the higher probability of severe.2.OAS1 rs1131454 loci gene polymorphism and EV71 sense There was no significant correlation between the risk of infection and the risk of infection.
【学位授予单位】：青岛大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：R725.1

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