一氧化氮参与调节PEG渗透胁迫下白三叶抗氧化酶活性及其基因表达

发布时间：2018-05-25 11:02

本文选题：白三叶 + 干旱胁迫　；参考：《草业学报》2017年04期

【摘要】：以‘拉丁诺’白三叶为材料,采用药理学实验,研究15%PEG-6000渗透胁迫下,离体叶片内源NO荧光产生及NO含量动态变化,NO清除剂及合成抑制剂对白三叶叶片抗氧化酶活性及其基因表达量的影响以及外源NO供体(SNP)对渗透胁迫的缓解效应。渗透胁迫可诱导白三叶叶片NO荧光产生,NO含量升高,提高抗氧化酶活性,上调抗氧化酶基因表达水平,而Hb、NR、NaVO_3和L-NAME则显著抑制了胁迫诱导的NO含量、抗氧化酶活性及其基因表达水平的提升;外源根施50μmol/L SNP促进了胁迫下叶片抗氧化酶活性的提高,并有效减缓了相对含水量的下降,抑制电解质渗透率(EL)和MDA含量的升高。以上结果表明,NO是白三叶响应渗透胁迫的重要信号分子,并可能通过调控氧化酶基因表达和提高抗氧化酶活性,以减轻渗透胁迫对白三叶幼苗的过氧化伤害。
[Abstract]:The white clover was used as the material, and the pharmacological experiment was used to study the osmotic stress of 15%PEG-6000. Effects of no scavenger and synthesis inhibitor on antioxidant enzyme activity and gene expression in white clover leaves and the effect of exogenous no donor SNPs on osmotic stress. Osmotic stress could induce the increase of no content, increase the activity of antioxidant enzyme and up-regulate the expression of antioxidant enzyme gene in white clover leaves, while the content of no was significantly inhibited by HbNR- NaVO3 and L-NAME. Exogenous root application of 50 渭 mol/L SNP promoted the increase of antioxidant enzyme activity in leaves under stress, and effectively slowed down the decrease of relative water content and inhibited the increase of electrolyte permeability (ELL) and MDA content. These results suggest that no is an important signal molecule in white clover response to osmotic stress and may reduce peroxidation injury of white clover seedlings by regulating the expression of oxidase gene and increasing the activity of antioxidant enzyme.
【作者单位】：四川农业大学动物科技学院草业科学系;
【基金】：国家自然科学基金(31372371) 四川省支撑项目(2013NZ0013)资助
【分类号】：S541.2

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