HOXA10及Integrin β3基因在子宫内膜异位症大鼠子宫内膜组织中的表达及意义

发布时间：2018-05-26 02:56

本文选题：子宫内膜异位症(EMs) + 不孕症　；参考：《世界最新医学信息文摘》2016年93期

【摘要】：目的:探讨HOXA10及Integrinβ3基因在子宫内膜异位症大鼠子宫内膜组织中的表达及意义。方法:分别取20只EMs大鼠子宫内膜标本及正常对照组大鼠子宫内膜标本,应用实时荧光定量PCR技术(FQ-PCR)、免疫组化从m RNA及蛋白质水平检测HOXA10及Integrinβ3基因在EMs大鼠以及对照组大鼠子宫内膜的表达。结果:HOXA-10及Integrinβ3基因m RNA及蛋白在EMs大鼠子宫内膜表达明显下降,相比具有统计学差异,P0.05。结论:HOXA-10及Integrinβ3基因在EMs症大鼠子宫内膜中的表达显著降低,可能是EMs不孕患者子宫内膜容受性降低,进而引起不孕的重要因素之一。
[Abstract]:Aim: to investigate the expression and significance of HOXA10 and Integrin 尾 3 genes in endometrium of endometriosis rats. Methods: the endometrial specimens of 20 EMs rats and normal control rats were collected. The expression of HOXA10 and Integrin 尾 3 genes in the endometrium of EMs rats and control rats were detected by using real-time fluorescence quantitative PCR technique. The expression of HOXA10 and Integrin 尾 3 genes in endometrium of EMs rats and control rats were detected by immunohistochemistry at the level of m RNA and protein. Results the expression of m RNA and protein of 10% HOXA-10 and Integrin 尾 3 gene were significantly decreased in the endometrium of EMs rats (P 0.05). Conclusion the expression of Integrin 尾 3 gene and 10% HOXA-10 gene is significantly decreased in endometrium of EMs rats, which may be one of the important factors leading to infertility in infertile women with EMs.
【作者单位】：南京医科大学附属常州市妇幼保健院生殖中心;
【分类号】：R711.71

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