冷藏期间苹果果实能量和NADP-苹果酸酶及基因表达的变化

发布时间：2018-05-26 08:43

  本文选题：苹果 + 机械冷藏　； 参考：《食品工业科技》2017年11期

【摘要】：为研究冷藏期间苹果果实能量水平和NADP-苹果酸酶(NADP-ME)及基因表达的变化情况,本研究以‘长富2’苹果果实为实验材料,定期测定果实硬度、可溶性固形物等品质指标和能量相关物质三磷酸腺苷(ATP)、二磷酸腺苷(ADP)、磷酸腺苷(AMP)含量和能荷变化及NADP-ME、琥珀酸脱氢酶(SDH)活性。结果显示,冷藏期间,‘长富2’果实ATP含量贮藏前60 d下降,而后恢复到贮藏初期水平,ADP和AMP贮藏前60 d稍有降低后,随时间延长显著增加;NADP-ME和琥珀酸脱氢酶(SDH)活性均随贮藏时间延长逐渐降低;辅酶Ⅱ(NADP+)和还原型辅酶Ⅱ(NADPH)含量在贮藏前120 d先增加后下降,与能荷变化相似;Mdcy ME1-Mdcy ME3基因表达虽在时间上有一定差异,但表现出相似的先增加后下降的趋势。冷藏期间苹果果实能量水平和利用效率发生了改变,且能量水平与NADP-ME活性及其基因表达在统计学上存在相关性。
[Abstract]:In order to study the changes of energy level, NADP- malate enzyme NADP-MEE and gene expression in apple fruit during cold storage, the fruit of 'Changfu 2'apple was used as experimental material, and the firmness of fruit was measured regularly. The content and energy charge of adenosine triphosphate (ATP), adenosine diphosphate (ADPN), adenosine monophosphate (AMPP), and the activities of NADP-MES and succinate dehydrogenase (SDH). The results showed that the ATP content of 'Changfu 2' fruit decreased 60 days before storage, and then returned to the initial storage level 60 days before storage, and then decreased slightly 60 days before storage. The activities of NADP-ME and succinate dehydrogenase SDH decreased gradually with the prolongation of storage time, and the contents of coenzyme 鈪，

本文编号：1936613