维生素D受体和降钙素受体基因多态性与新疆地区汉族女性人群骨密度的相关性

发布时间：2018-05-26 11:38

本文选题：维生素D受体基因多态性 + 降钙素受体基因多态性　；参考：《广东医学》2017年09期

【摘要】：目的研究维生素D受体(VDR)和降钙素受体(CTR)基因多态性与新疆地区汉族女性人群骨密度(BMD)的关系,探讨原发性骨质疏松症发病的遗传易感因素。方法采用聚合酶链反应(PCR-RFLP)限制性片段长度多态性技术,对336例新疆地区汉族女性人群的VDR和CTR基因进行多态性分析,双能X线吸收法测定受试者L_(2~4)、Ward's三角、大粗隆、股骨干4个部位的BMD值,比较不同基因型各部位BMD值的差异。结果(1)336例受试者VDR、CTR受体基因型频率分布均符合Hardy-Weinberg定律,90例骨质疏松组与246例非骨质疏松组VDR与CTR基因型分布频率差异无统计学意义(P0.05)。(2)年龄与不同部位BMD值之间呈负相关(P0.05),体质指数与BMD值之间呈正相关(P0.05),在将年龄和体质指数进行校正后发现非骨质疏松组中CTR CT基因型在Ward's三角的BMD值高于CC基因型(P0.05);VDR Bb基因型在L_(2~4)、Ward's三角部位BMD均低于bb型(P0.05),Bb基因型的BMD值在大粗隆、股骨干部位较bb型有降低趋势(P0.05);VDRCTR复合基因CCBb型的BMD值最低。结论年龄与BMD值变化密切相关;体质指数是新疆汉族女性非骨质疏松的保护性因素,适当增加体重对防治骨质疏松有积极的意义;VDR Bb基因型与BMD降低密切相关;VDR-CTR复合基因CCBb型可作为预测新疆地区女性发生骨质疏松危险性的遗传学标志。
[Abstract]:Objective to study the relationship between the polymorphism of vitamin D receptor (VDR) and calcitonin receptor (CTRR) gene and bone mineral density (BMD) in Han women in Xinjiang, and to explore the genetic predisposing factors of primary osteoporosis. Methods Polymerase chain reaction PCR-RFLP restriction fragment length polymorphism (RFLP) technique was used to analyze the polymorphism of VDR and CTR genes in 336 Han women in Xinjiang. The BMD values of four parts of the femoral shaft were compared with the BMD values of different genotypes. Results the frequency distribution of VDR and CTR genotypes in 336 subjects were in accordance with Hardy-Weinberg 's law. There was no significant difference between VDR and CTR genotypes in 90 cases of osteoporosis and 246 cases of non-osteoporosis. There was a positive correlation between body mass index (BMI) and BMD value. After adjusting age and BMI, it was found that the BMD value of CTR CT genotype in Ward's triangle was higher than that of CC genotype in Ward's triangle. The BMD value of BMD was lower than that of BB genotype P0.05Bb. The BMD value of the CCBb gene of the femoral shaft was lower than that of the BB type. Conclusion Age is closely related to the change of BMD, and BMI is the protective factor of non-osteoporosis in Han women in Xinjiang. Proper weight gain has positive significance for prevention and treatment of osteoporosis. VDR-CTR complex gene CCBb type can be used as a genetic marker to predict the risk of osteoporosis in women in Xinjiang area. The VDR-CTR genotype is closely related to the decrease of BMD.
【作者单位】：石河子大学医学院第一附属医院骨科中心关节外科;中国人民解放军第四七四医院药剂科;
【基金】：国家自然科学基金资助项目(编号:81360451,81660374) 兵团科技领军人才专项基金(编号:2016BC001) 兵团骨科创新团队专项(编号:2014CC002)
【分类号】：R580

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