光受体和OsPIN1基因家族表达对水稻根负向光性的影响

发布时间：2018-05-26 11:44

本文选题：水稻 + 光受体　；参考：《河北农业大学学报》2017年03期

【摘要】：为了探讨根尖光受体及生长素极性运输在水稻根负向光性中的作用,以超表达OsPIN1b转基因和野生型水稻(‘中花11’)种子根尖为材料,在根尖受单侧光照不同时间后,提取根尖RNA,反转录合成cDNA,通过半定量PCR检测3种光受体和OsPIN1基因家族4个基因表达差异,用激光共聚焦电子显微镜观察根尖融合表达蛋白OsPIN1b-GFP的定位,测量根负向光性弯曲程度。结果发现:用单侧光照射后,转基因与野生型水稻根尖光受体基因表达量均明显提高,OsPIN1基因家族4个基因表达量也得到提高,但转基因水稻的这几个基因的表达量较野生型高,在转基因水稻根尖发现细胞向光侧的OsPIN1b-GFP荧光强度明显弱于背光侧,水稻根负向光性角度也显著大于野生型。结果说明,水稻根尖受到单侧光照射后,提高了光受体和IAA极性输出载体基因的表达量,促进IAA极性运输,诱导根尖负向光性形成,由于转基因水稻的相关基因表达量高于野生型,所以根尖的负向光性弯曲角度明显大于野生型。
[Abstract]:In order to investigate the role of photoreceptor and polar transport of auxin in the negative light of root of rice, the overexpressed OsPIN1b transgenic and wild type rice 'Zhonghua 11') seed root tip were used as materials, and the root tip was exposed to unilateral illumination for different time. Root tip RNAs were extracted, reverse transcripted and synthesized into cDNAs. The difference of expression of four genes in three kinds of photoreceptors and OsPIN1 gene families was detected by semi-quantitative PCR. The localization of OsPIN1b-GFP, a fusion protein expressed in root tip, was observed by confocal electron microscope, and the degree of negative photocurving of root was measured. The results showed that the expression of light receptor genes in root tip of transgenic rice and wild type rice were significantly increased after irradiation with unilateral light, but the expression of these genes in transgenic rice was higher than that in wild type, but the expression of four genes in OsPIN1 gene family was higher than that in wild type rice. The fluorescence intensity of OsPIN1b-GFP in the light side of the transgenic rice root tip was obviously weaker than that in the backlight side, and the negative light angle of rice root was significantly higher than that of the wild type. The results showed that the expression of photoreceptor and IAA polarity output vector gene was increased, IAA polarity transport was promoted, and the formation of negative light of root tip was induced after the root tip was irradiated by unilateral light. Because the relative gene expression of transgenic rice was higher than that of wild type, the negative light bending angle of root tip was obviously higher than that of wild type.
【作者单位】：江苏理工学院化学与环境工程学院;绍兴文理学院生命科学学院;
【基金】：浙江省自然科学基金(LY14C130003;LY16C130004) 国家自然科学基金(31071353) 江苏省产学研前瞻联合性研究基金(BY2015028-04)
【分类号】：S511

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