高坡猪肌肉生长抑制素基因多态性及其与肉质性状的相关性分析

发布时间：2018-05-27 04:42

本文选题：高坡猪 + MSTN基因　；参考：《中国畜牧兽医》2017年04期

【摘要】：为了探讨肌肉生长抑制素(myostatin,MSTN)基因的多态性对高坡猪肉质性状(水分、粗脂肪、粗蛋白质、粗灰分、眼肌面积、大理石纹、pH、肉色、嫩度、滴水损失、失水率)的影响,试验以MSTN基因作为肉质性状候选基因,以10月龄的50头高坡猪为研究对象,采用PCR扩增产物直接测序的方法对高坡猪MSTN基因3个外显子的单核苷酸多态性(SNP)进行研究,运用SPSS 20.0软件分析MSTN基因SNP与肉质性状的关联性。结果表明,仅在高坡猪MSTN基因第3外显子63bp处检测到1个C/T突变位点,该突变为同义突变,未引起编码氨基酸的改变。基因型分析发现,仅有3个个体在该位点发生突变,CC基因型为优势基因型,C为优势等位基因,无TT纯合基因型。经χ~2适合性检验分析,该SNP在研究群体中处于Hardy-Weinberg平衡状态(P0.05)。群体遗传参数分析发现,C63T标记位点的杂合度(He)相对较低,表明其在高坡猪群体中的变异较小;就多态信息含量(PIC)而言,该位点属于低度多态(PIC0.25),说明该遗传标记能够提供少量的遗传信息。将该位点的不同基因型与肉质性状指标进行关联分析表明,所有肉质性状指标在不同基因型间差异均不显著(P0.05)。综上所述,MSTN基因外显子在高坡猪中存在多态性,但变异较少,相对保守,能否作为高坡猪肉质性状的遗传标记有待于扩大样本数量进一步研究。
[Abstract]:To investigate the effect of MSTN gene polymorphism on the quality traits (water, crude fat, crude protein, crude ash, eye muscle area, marbling pH, meat color, tenderness, drip loss, water loss rate) of high slope pork, and to investigate the effect of MSTN gene polymorphism on the quality traits (water content, crude fat, crude protein, crude ash, eye muscle area, marbling pH, meat color, tenderness, drip loss and water loss rate). MSTN gene was used as candidate gene for meat quality traits and 50 Gaopo pigs aged 10 months were used to study the single nucleotide polymorphism (SNP) of three exons of MSTN gene by direct sequencing of PCR amplification products. The relationship between MSTN gene SNP and meat quality traits was analyzed by SPSS 20.0 software. The results showed that only one C / T mutation was detected at 63bp of exon 3 of MSTN gene in Gaopo pig. The mutation was synonymous and did not cause the change of encoding amino acid. Genotypic analysis showed that only 3 individuals had mutation at this locus and the CC genotype was the dominant allele, and no TT homozygous genotype. According to 蠂 ~ 2 fitness test, the SNP was in the Hardy-Weinberg equilibrium state in the study population (P 0.05). The analysis of population genetic parameters showed that the heterozygosity of C63T marker was relatively low, which indicated that the variation was small in Gaopo pig population, and the polymorphic information content (PICs) was higher than that in Gaopo pig population. This locus belongs to low polymorphic PIC0.25, which indicates that the genetic marker can provide a small amount of genetic information. The correlation analysis between different genotypes of this locus and meat quality traits showed that there was no significant difference in all flesh-quality traits among different genotypes (P 0.05). To sum up, the exon of MSTN gene is polymorphic in Gaopo pigs, but the variation is relatively conservative, so whether it can be used as a genetic marker for the quality traits of high slope pork needs to be further studied.
【作者单位】：贵州大学动物科学学院高原山地动物遗传育种与繁殖教育部重点实验室;正安县畜牧产业发展办公室;贵州省安顺市畜牧技术推广站;
【基金】：基金项目:高坡猪种质资源利用与高效养殖模式研究(黔科合NY字[2010]3050号)
【分类号】：S828

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