污泥臭氧氧化处理过程中活菌抗药基因丰度的消减

发布时间：2018-05-27 07:38

本文选题：活性污泥 + 臭氧氧化　；参考：《环境工程学报》2017年05期

【摘要】：臭氧氧化技术是一种广泛应用的污泥减量技术,然而臭氧处理能否对污泥中的抗生素抗药基因进行有效消减还不清楚。采用单叠氮溴化丙锭(propidium monoazide,PMA)预处理结合定量PCR(qPCR)方法对污泥臭氧减量过程中不同臭氧消耗量下活性污泥活菌中的四环素、氨基糖苷和大环内酯3大类共21种抗药基因的变化进行了研究。结果表明:臭氧氧化可以有效消减活性污泥活菌中21种抗药基因的绝对丰度(每毫升污泥的抗药基因拷贝数),在臭氧消耗量0.31 g·g~(-1)(TSS)情况下抗药基因总量降低了75.44%;尽管绝对丰度下降,污泥臭氧氧化处理过程中大量抗药基因的相对丰度(抗药基因拷贝数与细菌16S rRNA基因拷贝数的比例)逐渐增加,表明臭氧处理后污泥中耐药菌占总活菌比例可能增加,具有一定的抗性传播风险;Ⅰ型整合子是抗药基因水平转移的重要遗传元件,其绝对丰度同样随着臭氧消耗量增加而降低,而相对丰度逐渐上升。
[Abstract]:Ozone oxidation is a widely used sludge reduction technology. However, it is not clear whether ozone treatment can effectively reduce antibiotic resistance genes in sludge. The tetracycline in activated sludge living bacteria was treated with propidium monoazide PMA pretreatment combined with quantitative PCRX qPCRduring the process of sludge ozone reduction. The changes of 21 resistant genes of aminoglycoside and macrolide were studied. The results showed that ozone oxidation could effectively reduce the absolute abundance of 21 resistant genes (copy number of drug resistance genes per milliliter of sludge), and the total amount of drug resistant genes decreased by 75.44% under ozone consumption of 0.31 g / ml TSSs. The absolute abundance of the tube drops, The relative abundance of a large number of drug-resistant genes (the ratio of copy number of drug resistance gene to 16s rRNA gene) gradually increased during ozonation treatment of sludge, indicating that the proportion of drug-resistant bacteria in total live bacteria may increase after ozone treatment. Type I integron is an important genetic element for horizontal transfer of drug resistance genes, and its absolute abundance also decreases with the increase of ozone consumption, while the relative abundance increases gradually.
【作者单位】：北京工业大学北京市水质科学与水环境恢复工程重点实验室;中国科学院生态环境研究中心环境水质学国家重点实验室;中国科学院大学;
【基金】：国家自然科学基金资助项目(21590814)
【分类号】：X703.1

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