当归多糖对点带石斑鱼Toll样受体22信号通路相关基因表达的影响

发布时间：2018-05-28 02:29

本文选题：点带石斑鱼 + 当归多糖　；参考：《大连海洋大学学报》2017年01期

【摘要】：为研究当归多糖Angelica sinensis polysaccharide(ASP)对点带石斑鱼Epinephelus malabaricus Toll样受体22(Toll-like receptor 22,TLR22)信号通路相关基因表达的影响,以当归多糖体外孵育点带石斑鱼头肾白细胞,采用实时荧光定量PCR(q PCR)方法检测了不同时间点(3、6、12、24、48 h)TLR22信号通路相关基因m RNA相对表达量的变化。结果表明:TLR22及与其接头的Toll样接头蛋白1(TIR-domaincontaining adaptor inducing interferon-β,TRIF)的表达量均随时间的延长逐渐升高,分别于24、12 h达到峰值;TRIF下游的干扰素调节因子(Interferon regulatory factor 3,IRF3)在24 h表达量达到最大值,此时主要组织相容性复合体(Major histocompatibility complex,MHC)与肿瘤坏死因子(Tumor necrosis factor-α,TNF-α)的表达量也开始到达峰值;白介素-8(Interleukin-8,IL-8)的表达量在48 h达到最高。研究表明:当归多糖可激活TLR22及其下游相关基因,并在免疫调节过程中起到重要作用。
[Abstract]:In order to study the effect of Angelica polysaccharide Angelica sinensis polysaccharide on the expression of genes related to the signal transduction pathway of Epinephelus malabaricus Toll like receptor 22(Toll-like receptor 22TLR22, Angelica polysaccharide was incubated with angelica polysaccharide with head kidney leukocytes of grouper in vitro. A real-time fluorescence quantitative PCR(q method was used to detect the relative expression of m RNA, a signal transduction pathway related gene m RNA, at different time points. The results showed that the expression of 1(TIR-domaincontaining adaptor inducing interferon- 尾 trif, a Toll like junction protein with TLR22, increased gradually with time, and reached the peak at 24 h after 24 h. The expression of Interferon regulatory factor 3 (IRF3), which was downstream of TLR22, reached its highest level at 24 h. At the same time, the expression of Major histocompatibility complex (MHC) and Tumor necrosis factor- 伪 (TNF- 伪) of major histocompatibility complex (MHC) began to reach its peak, and the expression of IL-8 (Interleukin-8IL-8) reached its highest level at 48 h. The results showed that Angelica polysaccharide could activate TLR22 and its downstream genes and play an important role in immune regulation.
【作者单位】：天津农学院水产学院天津市水产与养殖生态重点实验室;
【基金】：国家自然科学基金资助项目(31270456,31402313) 国家“星火计划”重大项目(2013GA610002) 天津市科技支撑计划重大项目(12ZCDZNC05900,13ZCZDNC00700) 国家农业科技成果转化项目(2014GB2A100528) 天津市应用基础与前沿技术研究计划项目(15JCZDJC33600,15JCZDJC34000)
【分类号】：S948

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