春秋两季不同地区猪源耐药大肠杆菌β-内酰胺酶及16SrRNA甲基化酶基因检测及分析

发布时间：2018-05-28 14:51

  本文选题：春秋两季 + 不同地区　； 参考：《中国农业科技导报》2017年05期

【摘要】：为了解新疆春秋两季不同地区耐药猪源大肠杆菌携带β-内酰胺酶和16S rRNA甲基化酶基因及其共存情况。通过PCR方法对克拉玛依地区142株春季猪源耐药菌、9株克拉玛依秋季猪源耐药菌、78株昌吉地区春季猪源耐药菌和52株昌吉地区秋季猪源耐药菌进行β-内酰胺酶(blaTEM、blaCMY-2、blaLAP-1、blaKPC、blaSHV和blaOXA)和16S rRNA甲基化酶(arm A和rmt B)基因检测,并对检出耐药基因的PCR产物进行测序。结果显示:克拉玛依春季猪源耐药菌携带的β-内酰胺酶基因为blaTEM(142/142,100%)、blaOXA(16/142,11.3%)、blaCMY-2(7/142,4.9%)和blaLAP-1(1/142,0.7%);克拉玛依秋季猪源耐药菌携带的β-内酰胺酶为blaTEM(9/9,100%)和blaOXA(1/9,11.1%),克拉玛依春秋两季猪源耐药菌均未检测出16S rRNA甲基化酶基因;昌吉地区春季猪源耐药菌携带的β-内酰胺酶基因为blaTEM(78/78,100%)、blaOXA(9/78,11.5%)、blaCMY-2(3/78,3.8%)和16S rRNA甲基化酶基因rmt B(1/78,1.3%);昌吉地区秋季猪源耐药菌携带的β-内酰胺酶基因为blaTEM(52/52,100%)、blaOXA(4/52,7.7%)、blaCMY-2(3/52,5.8%)和blaSHV(1/52,1.9%),未检测出16S rRNA甲基化酶基因。上述结果表明春秋两季不同地区猪源大肠杆菌blaTEM检出率均为100%;其次不同地区春季猪源大肠杆菌对blaOXA检出率均高于秋季猪源大肠杆菌。产生β-内酰胺酶及16S rRNA甲基化酶基因是猪源菌对β-内酰胺类及氨基糖苷类抗菌药物耐药的主要原因之一,养殖场应合理使用抗菌药物,加强对产生β-内酰胺酶及16S rRNA甲基化酶基因的监控。
[Abstract]:In order to understand the coexistence of 尾 -lactamase and 16s rRNA methylase gene in Escherichia coli from different areas of Xinjiang in spring and autumn. Application of 尾 -lactamase (尾 -lactamase) to 142 strains of spring porcine drug-resistant bacteria in Karamay region, 9 strains of klamayi autumn porcine drug-resistance bacteria, 78 strains of spring porcine drug-resistant bacteria in Changji area and 52 strains of autumn porcine drug-resistant bacteria in Changji area were used to detect 尾 -lactamase (尾 -lactamase) blaCMY-2 blaLAP-1blaKPCblaSHV and 尾 -lactamase (尾 -lactamase) blaCMY-2 blaLAP-1KPCblaSHV. Detection of blaOXA and 16s rRNA methylases ARMA and rmt B, The PCR products of drug resistance gene were sequenced. The results showed that the 尾 -lactamases carried by resistant strains from spring pigs in Karamay were as follows: blaTEM142 / 142100 because of blaTEM142P / 142100, 16 / 142OXAX / 16 / 1422.3X / 7 / 7 / 1424.9b) and blaLAP-1 / 1 / 1420.70.70.The 尾 -lactamases carried by resistant strains from pigs in Karamay fall were TEblaM9r9100m) and blaOXA1 / 911.1x, respectively, and the resistant bacteria from pig origin were not detected in Karamayi in spring and autumn. The 尾 -lactamases carried by drug-resistant bacteria from pig origin of Karamayi in autumn were TEblaM9 / 9100) and blaOXA1 / 9 / 9 / 11 / 1Q, respectively. 16s rRNA methylase gene was obtained. 鏄屽悏鍦板尯鏄ュ�ｇ尓婧愯�愯嵂鑿屾惡甯︾殑尾-鍐呴叞鑳洪叾鍩哄洜涓篵laTEM(78/78,100%),blaOXA(9/78,11.5%),blaCMY-2(3/78,3.8%)鍜，

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