子痫前期宫内环境导致子代大鼠海马HPA轴相关基因表达改变

发布时间：2018-05-28 20:18

本文选题：子痫前期 + 子代大鼠　；参考：《复旦学报(医学版)》2017年04期

【摘要】：目的研究子痫前期宫内环境对子代SD大鼠海马下丘脑-垂体-肾上腺(hypothalamic-pituitaryadrenal,HPA)轴相关基因表达的影响。方法通过给妊娠第14天SD大鼠连续皮下注射亚硝基左旋精氨酸甲酯(L-NAME)至分娩的方法建立子痫前期大鼠动物模型,对照组予生理盐水1 mL皮下注射。用酶联免疫检测(ELISA)方法检测新生子代大鼠血皮质酮(corticosterone,CORT)浓度。用Real-time PCR方法和Western bolt分别检测8周龄子代大鼠海马组织糖皮质激素受体(glucocorticoid receptor,GR)、促肾上腺皮质激素释放激素(corticotropin releasing hormone,CRH)、CRH受体1(CRH receptor 1,CRHR1)和IL-6的mRNA水平及其蛋白质水平。结果与正常子代新生鼠相比,子痫前期子代新生鼠血CORT浓度升高(P0.05)。与正常子代鼠相比,子痫前期青少年子代鼠海马GR、CRH和CRHR1 mRNA和蛋白质水平均增加(P0.05),炎性因子IL-6mRNA和蛋白质水平均增加(P0.05)。结论子痫前期宫内环境影响子代大鼠海马HPA轴相关基因的表达,在子代学习记忆能力受损中起作用。
[Abstract]:Objective to study the effect of intrauterine environment of preeclampsia on the expression of genes related to the axis of hypothalamic-pituitary-adrenal gland (HPAA) in rat hippocampus. Methods the preeclampsia rat model was established by subcutaneous injection of L-NAME-nitroso-L-arginine methyl ester into labor on the 14th day of pregnancy, and the control group was subcutaneously injected with 1 mL normal saline. The plasma corticosterone (Cort) concentration in newborn rats was detected by Elisa. The levels of glucocorticoid receptor (Glucocorticoid receptor), corticotropin releasing hormone (corticotropin releasing CRH receptor 1(CRH receptor 1CRHR1) and IL-6 and their protein levels in hippocampus of 8-week-old rats were detected by Real-time PCR and Western bolt, respectively. Results compared with normal newborn mice, the serum CORT concentration of preeclampsia newborn rats was higher than that of normal offspring. Compared with normal offspring, the levels of CRH, CRHR1 mRNA and protein in hippocampal GRG of juvenile preeclampsia rats were increased, while the levels of IL-6mRNA and protein of inflammatory factors were increased. Conclusion the intrauterine environment of preeclampsia affects the expression of HPA axis related genes in the hippocampus of the offspring and plays an important role in the impairment of learning and memory ability of the offspring.
【作者单位】：复旦大学附属妇产科医院产科;
【基金】：国家自然科学基金(81571460,81270712)~~
【分类号】：R714.244

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