准噶尔无叶豆EsDREB2B基因在烟草中的抗逆功能分析

发布时间：2018-05-29 01:56

本文选题：DREB转录因子 + 准噶尔无叶豆　；参考：《分子植物育种》2016年02期

【摘要】：DREB(dehydration responsive element binding protein)转录因子在植物响应非生物胁迫上具有重要作用,是颇具潜力的植物抗逆育种候选基因。本研究旨在通过烟草体系,验证前期从准噶尔无叶豆中克隆的Es DREB2B基因在种子萌发和成苗成长阶段的抗逆功能,并初步探究其调控的下游靶基因。结果表明:转基因株系在干旱、高盐、冷和热胁迫处理下的萌发率都高于野生型烟草,其中盐和热胁迫下萌发率差异尤为显著;两月大转基因烟草与野生型植株进行干旱、高盐、冷和热胁迫处理后,转基因较野生型植株叶片萎蔫,黄化和伤害程度较轻,在热胁迫下差异最为显著;恢复培养后,大多野生型烟草表现为枯死状态,转基因植株则叶片萎蔫减轻并开始生长,并且转基因烟草存活率显著高于野生型,均达到了野生型的3~4倍。ERD10B和Hsp70基因在四种胁迫后的转基因烟草中的表达量均较野生型高,Hsp90在热胁迫下较野生型表达量升高,而Mn SOD,P5CS等基因的表达量变化不显著。以上结果表明,Es DREB2B基因能提高烟草抗干旱、高盐、冷和热胁迫的能力,是具有潜力的抗逆分子育种候选基因。
[Abstract]:DREB(dehydration responsive element binding protein) transcription factors play an important role in plant response to abiotic stress and are potential candidate genes for plant stress resistance breeding. The purpose of this study was to verify the stress resistance of es DREB2B gene cloned from leafless legumes from Junggar in the early stage of seed germination and seedling growth, and to explore the downstream target genes. The results showed that the germination rate of transgenic lines under drought, high salt, cold and heat stress was higher than that of wild type tobacco, especially under salt and heat stress. Under high salt, cold and heat stress, the leaf wilting, yellowing and injury degree of transgenic plants were lighter than those of wild type plants, and the difference was most significant under heat stress. The leaves wilted and began to grow, and the survival rate of transgenic tobacco was significantly higher than that of wild type. The expression of ERD10B and Hsp70 genes in transgenic tobacco was higher than that of wild type Hsp90 under heat stress, but there was no significant change in the expression of mn SODN P5CS and other genes. These results indicated that the DREB2B gene could enhance the ability of resistance to drought, high salt, cold and heat stress, and was a potential candidate gene for stress resistance molecular breeding.
【作者单位】：中国科学院干旱区生物地理与生物资源重点实验室中国科学院新疆生态与地理研究所;中国科学院大学;
【基金】：新疆维吾尔自治区重大专项项目(201330122-3)资助
【分类号】：Q943.2

【相似文献】