星油藤关键酶基因PvFAD3启动子的克隆及表达激活分析

发布时间：2018-05-29 02:00

  本文选题：星油藤 + α-亚麻油酸　； 参考：《植物生理学报》2017年01期

【摘要】：α-亚麻油酸(α-linolenic acid,ALA)对防治脂类代谢失调而引起的肥胖、心血管疾病、高血脂、高血压等慢性疾病具有重要的生理功能。然而,大部分油料作物种子ALA的含量都很低,提高种子ALA的含量一直是油料作物品质改良的重点内容之一。星油藤(Plukenetia volubilis)种子油中富含ALA,是一种具有重要开发前景的油料植物,解析其种子高效累积ALA的分子机理不仅能为星油藤的遗传改良提供理论依据,而且能够为提高油料作物种子ALA含量的遗传育种提供理论参考。前期的研究发现星油藤种子高效累积ALA主要是由于关键酶基因Pv FAD3高度表达的结果。为了进一步理解星油藤种子在发育过程中Pv FAD3的被调控机理,本研究利用染色体步移法克隆了Pv FAD3的启动子,并且利用生物信息学分析、鉴别了启动子潜在的关键调控元件,并重点围绕可能调控Pv FAD3基因表达的b ZIP124转录因子,通过酵母单杂交和烟草叶片启动子激活实验,验证了Pv FAD3的表达受b ZIP124转录因子的调控。该研究为理解植物种子高效累积ALA的分子机理提供了新的证据。
[Abstract]:伪-linolenic acid (伪-linolenic acid ALAs) plays an important physiological role in the prevention and treatment of obesity, cardiovascular disease, hyperlipidemia, hypertension and other chronic diseases caused by dyslipidemia. However, the content of ALA in seed of most oil crops is very low, and increasing the content of seed ALA is always one of the key contents of oil crop quality improvement. The seed oil of Plukenetia volubilis (Plukenetia volubilis) is rich in ALAs and is a promising oil plant. The molecular mechanism of ALA accumulation in its seeds can not only provide a theoretical basis for genetic improvement. Moreover, it can provide theoretical reference for genetic breeding of improving ALA content of oil crop seeds. In previous studies, it was found that the high accumulation of ALA in seeds was mainly due to the high expression of the key enzyme gene PV FAD3. In order to further understand the regulated mechanism of PV FAD3 during development, the promoter of PV FAD3 was cloned by chromosome step method, and the potential key regulatory elements of the promoter were identified by bioinformatics analysis. The b ZIP124 transcriptional factors which may regulate the expression of PV FAD3 gene were focused on. The results showed that the expression of PV FAD3 was regulated by b ZIP124 transcription factors through yeast single hybridization and tobacco leaf promoter activation experiments. This study provides new evidence for understanding the molecular mechanism of ALA accumulation in plant seeds.
【作者单位】： 中国科学院昆明植物研究所;中国科学院大学;周口师范学院生命科学系;
【基金】：国家自然科学基金(31571709) 云南省应用基础自然科学基金(2014FB172)~~
【分类号】：Q943.2;S565.9
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本文编号：1949062