二穗短柄草钙依赖型蛋白激酶BdCDPK4基因的克隆及其编码蛋白质的生物信息学分析

发布时间：2018-05-29 14:25

本文选题：CDPKs + BdCDPK　；参考：《浙江农业学报》2017年02期

【摘要】：二穗短柄草(Brachypodium distachyon L.)因与小麦、大麦等有很高的同源性,作为新型禾本科模式生物吸引了越来越多的研究者。试验采用基因克隆技术分离了二穗短柄草Bd CDPK4基因的编码区序列,并采用生物信息学方法分析了该基因及其编码的蛋白质的序列特征、结构和功能。结果表明,分离了BdCDPK4基因1 851 bp的cDNA序列(Gen Bank登录号为XM_003559516),编码区长度为1 752 bp,编码583个氨基酸,分子量为64.78 ku,等电点为9.12,含有N端可变区、蛋白激酶区、自抑制区、EF手型结构、类似钙调素等结构域。亚细胞定位预测位于叶绿体和液泡上。进化树分析结果显示,该蛋白与小麦Ta CDPK19蛋白的同源性最近。根据各物种间CDPKs蛋白的高同源性,结合小麦TaCDPK19基因被高盐和小麦白粉病菌Blumeria graministritici(Bgt)诱导,推测Bd CDPK4基因也可能参与逆境胁迫下的信号传导途径,在抗逆等生物学过程中发挥重要功能。
[Abstract]:Two Brachypodium distachyon L., which has high homology with wheat and barley, has attracted more and more researchers as a new type of gramineous model organisms. The sequence of the encoding region of the Bd CDPK4 gene of two spikes of short stalks was isolated by gene cloning technique, and the bioinformatics method was used to analyze the gene and its coding. The sequence characteristics, structure and function of the code protein showed that the cDNA sequence of the BdCDPK4 gene 1851 BP (Gen Bank login number was XM_003559516), the length of the coding region was 1752 BP, the encoding 583 amino acids, the molecular weight of 64.78 Ku, and the isoelectric point 9.12, containing the N terminal variable region, the protein kinase area, the self inhibition zone, the EF hand type structure, similar calcium. The subcellular localization prediction was located in the chloroplast and the vacuole. The evolutionary tree analysis showed that the protein was closest to the wheat Ta CDPK19 protein. According to the high homology of the CDPKs protein among the species, the TaCDPK19 gene of wheat was induced by high salt and Blumeria graministritici (Bgt) of wheat powdery mildew, and Bd CDPK4 was conjectured. Genes may also participate in signal transduction pathways under stress, and play important roles in biological processes such as stress resistance.
【作者单位】：武汉职业技术学院生物工程学院应用生物技术研究所;科技部国际科技合作基地(基因工程)分子生物物理学教育部重点实验室华中科技大学生命科学与技术学院;
【基金】：高校博士点专项科研基金(20120142110075) 湖北省教育厅自然科学研究计划项目(B2016581) 河南省教育厅自然科学研究计划项目(2009B210005) 武汉职业技术学院校级博士科研基金(2016BS001)
【分类号】：Q943.2

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