ESR基因多态性与湖羊产羔性能的关系

发布时间：2018-05-29 15:51

  本文选题：湖羊 + ESR基因　； 参考：《江苏农业科学》2017年13期

【摘要】：利用PCR-单链构象多态性(PCR-SSCP)技术及基因测序技术对湖羊进行了ESR基因的多态性检测及其与湖羊产羔数的统计分析。测序结果表明,等位基因G与C相比发生了1处碱基突变(G363C)。ESR基因在湖羊中存在多态性(CC、CG和GG);湖羊中C等位基因的频率为0.743,G等位基因的频率为0.257。CC、CG、GG基因型频率分别为0.564、0.357、0.079。湖羊CG、GG、CC基因型的平均产羔数分别为1.98、1.80、1.96只。数据分析结果表明,ESR基因的多态性对湖羊产羔数的影响差异不显著(P≥0.05)。
[Abstract]:PCR- single strand conformation polymorphism (PCR-SSCP) technique and gene sequencing technique were used to detect the polymorphism of ESR gene in Hu sheep and its statistical analysis with the litter size of Hu sheep. The sequencing results showed that there was a base mutation of G363CU. ESR gene in Hu sheep, and the frequency of C allele was 0.743 G allele in Hu sheep, and the frequency of C allele in Hu sheep was 0.257.CCN CGG GG genotype frequency was 0.5640.357GG genotype frequency was 0.5640.3570.79, respectively. The average number of lambs for the GGCCC genotypes of Hu sheep was 1.98 (1.80) and 1.96 respectively. The results of data analysis showed that the polymorphism of ESR gene had no significant difference in litter size of Hu sheep (P 鈮，

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