茶树几丁质酶基因的克隆及其在干旱胁迫下的表达分析

发布时间：2018-05-29 16:40

本文选题：茶树 + 几丁质酶　；参考：《热带作物学报》2017年05期

【摘要】：以铁观音茶树叶片为材料,利用逆转录PCR及RACE法,克隆了茶树几丁质酶基因Cs Chi(Gen Bank登录号为KR078345)。Cs Chi基因的c DNA全长为1 192 bp,包含972 bp的开放阅读框(ORF),编码323个氨基酸。生物信息学分析结果表明,Cs Chi蛋白的分子量为34.33 ku;理论等电点p I为8.44;原子组成为C1 519H2 285N413O464S18,总原子数为4 699;蛋白质结构分析显示该蛋白有6个蛋白的跨膜区域,属于跨膜蛋白;存在于细胞外;没有卷曲螺旋结构存在;Cs Chi基因编码的蛋白属于糖苷水解酶19家族,含有保守的Cht BD1结构域,与溶菌酶的保守结构域类似,可能兼具几丁质酶活性和溶菌酶活性,q PCR定量分析结果显示在不同干旱胁迫处理下茶树的Cs Chi基因的表达量,与对照组相比有所增加。推测Cs Chi基因在茶树干旱等逆境胁迫中起重要作用。
[Abstract]:Using leaf leaves of Tieguanyin tea as material, using reverse transcription PCR and RACE method, the c DNA of tea tree chitinase gene Cs Chi(Gen Bank accession number is 1 192 BP, containing 972 BP open reading frame, encoding 323 amino acids. The results of bioinformatics analysis showed that the molecular weight of Cs Chi protein was 34.33ku; the theoretical isoelectric point Pi was 8.44; the atomic composition was C1 519H2 285N413O464S18, the total atomic number was 4699.The protein structure analysis showed that the protein had six transmembrane regions and belonged to transmembrane protein. The protein encoded by Cs Chi gene is a family of glycoside hydrolase 19, which contains conserved Cht BD1 domain, similar to the conserved domain of lysozyme. The results of Q PCR quantitative analysis of chitinase activity and lysozyme activity showed that the expression of Cs Chi gene in tea plants under different drought stress was higher than that in the control group. It is inferred that Cs Chi gene plays an important role in the stress of tea plants such as drought.
【作者单位】：福建农林大学园艺学院;福建农林大学园艺植物生物工程研究所;福州市农业局;
【基金】：福建省自然科学基金(No.2013J01085) 福建省重大科技专项(No.2015NZ0002-1) 福建省教育厅计划项目(No.JA15143)
【分类号】：S571.1

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