林麝生长激素基因的克隆与生物信息学分析

发布时间：2018-05-29 20:29

本文选题：林麝 + GH基因　；参考：《西北林学院学报》2016年06期

【摘要】：了解林麝生长激素(GH)基因序列结构特点,为进一步研究GH基因的结构功能遗传变异规律及其与生长性能的关系提供科学依据。根据GenBank上已公布的绵羊全基因组GH基因序列设计合成4对特异性引物,以林麝基因组DNA为模板进行PCR扩增,将所获得的序列拼接,获得林麝GH基因CDS区序列,采用ExPASy等分子生物学工具对林麝GH蛋白的理化性质、二级结构、蛋白功能等进行预测。结果表明,林麝GH基因CDS区全长657bp(GenBank登录序列号为KU296865),编码218个氨基酸。α-螺旋结构和卷曲结构构成了林麝GH蛋白二级结构的骨架,为跨膜蛋白,同时第1~26位氨基酸为信号肽,第27~218位氨基酸残基为生长激素结构域。进化分析结果表明,林麝与羊的关系最为接近,在分子水平上符合物种进化理论。本研究成功克隆了林麝GH基因CDS区序列,其序列保守性强,为下一步林麝GH基因的表达调控、进化和多态性分析奠定了基础。
[Abstract]:To understand the sequence structure of growth hormone (GH) gene of musk deer, and to provide scientific basis for further study on the genetic variation of GH gene structure and function and its relationship with growth performance. According to the published GH gene sequence of sheep genome on GenBank, four pairs of specific primers were designed and synthesized. The genomic DNA of forest musk deer was used as template for PCR amplification. The obtained sequence was spliced together to obtain the sequence of CDS region of forest musk deer GH gene. The physicochemical properties, secondary structure and protein function of GH protein in forest musk deer were predicted by molecular biological tools such as ExPASy. The results showed that the full-length 657bp(GenBank accession number of the CDS region of the GH gene of the forest musk deer was KU296865, encoding 218 amino acids. 伪 -helix structure and curl structure formed the framework of the secondary structure of GH protein of musk deer, which was transmembrane protein, and the first 26 amino acid was a signal peptide. The amino acid residues at position 27 ~ (218) are the growth hormone domain. The results of evolutionary analysis show that the relationship between forest musk deer and sheep is the most close, and it accords with the theory of species evolution at the molecular level. In this study, the CDS sequence of the GH gene was cloned successfully, and the sequence was highly conserved, which laid a foundation for the regulation, evolution and polymorphism analysis of GH gene in the next step.
【作者单位】：四川养麝研究所繁育研究室;重庆市药物种植研究所药用动物研究室;
【基金】：国家自然科学基金(81503188) 重庆市卫计委中医药科技项目(ZY20150225) 四川省基本科研业务费项目(2016YSKY0068)
【分类号】：S865.41

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