马身猪MEF2A基因4种可变剪接体的克隆和生物信息学分析

发布时间：2018-05-29 21:45

本文选题：马身猪 + MEFA基因　；参考：《中国畜牧兽医》2017年04期

【摘要】：本研究旨在克隆马身猪肌细胞增强因子2A(myocyte enhancer factor 2A,MEF2A)基因的不同剪接体类型。依据转录组测序对可变剪接的预测结果,试验扩增MEF2A基因第5~8外显子之间的编码区,用生物信息学软件分析了马身猪MEF2A基因不同剪接体的序列特性,预测保守结构域,构建系统进化树,比对不同物种MEF2A的氨基酸同源性。结果显示,获得了4个MEF2A基因的剪接体,MEF2A1的第5~8外显子正常剪接;MEF2A2缺失了第5外显子,第6外显子的5′端多出138bp,第7外显子的3′端多出102bp;MEF2A3缺失了第5外显子,第6外显子的5′端多出138bp;MEF2A4第7外显子的3′端多出102bp。插入的138bp序列编码的蛋白质中存在1个保守结构域HJURP_C,这可能与MEF2A参与肝细胞纤维化作用有关。MEF2A1和MEF2A4与猪MEF2A1(GenBank登录号:NP_001090890.1)同属于一个亚群,同源性高达98.9%,MEF2A2和MEF2A3与猪MEF2A2(GenBank登录号:NP_001093168.1)同属于一个亚群,同源性分别为98.2%和98.9%。本试验成功克隆了4个MEF2A基因的剪接体,为进一步研究其蛋白功能奠定基础。
[Abstract]:The aim of this study was to clone different splicing types of myocyte enhancer 2A(myocyte enhancer factor 2 An MEF 2 A gene. Based on the prediction of variable splicing by transcriptome sequencing, the coding region between exon 5 of MEF2A gene was amplified. The sequence characteristics of different splicing bodies of MEF2A gene were analyzed by bioinformatics software, and the conserved domain was predicted. Phylogenetic tree was constructed to compare the amino acid homology of MEF2A in different species. The results showed that exon 5, exon 5 in exon 5, exon 5 The 5'end of exon 6 was 102bpmore than the 3'end of exon 7 of MEF2A4. There is a conserved domain HJURP C in the protein encoded by the inserted 138bp sequence, which may be related to the involvement of MEF2A in hepatocyte fibrosis. MEF2A1 and MEF2A4 belong to the same subgroup as MEF2A1(GenBank accession number: NP001090890.1. The homology of MEF2A2 and MEF2A3 were 98.2% and 98.9%, respectively, which belonged to the same subgroup as Pig MEF2A2(GenBank accession number: NP00103168.1). In this study, four splicing bodies of MEF2A gene were cloned successfully, which laid a foundation for further study of its protein function.
【作者单位】：山西农业大学动物科技学院;
【基金】：山西省科技创新重点团队项目(201605D131045-24) 三晋学者项目(2016) 山西省农业科技攻关项目(20140311020-5) 山西省高等学校优秀青年学术带头人项目
【分类号】：Q78;S828

【相似文献】