小檗碱对猪卵母细胞体外成熟过程miRNA及脂质代谢相关基因的影响

发布时间：2018-05-30 07:20

本文选题：Ber + miRNA　；参考：《中国畜牧杂志》2017年10期

【摘要】：本试验旨在探明小檗碱(Berberine,Ber)对猪卵母细胞体外成熟的作用及其对miRNA表达差异和脂滴代谢相关基因的影响。吸取法获取卵丘-卵母细胞复合体,0.1%透明质酸酶脱除颗粒细胞的未成熟卵母细胞为GV组,以TCM-199培养液为体外成熟液作为对照组,添加中药单体成分Ber为Ber组,观察卵母细胞成熟效果,基因芯片技术分析Ber对猪卵母细胞体外成熟过程中miRNA的差异变化,尼罗红染色测定脂滴含量变化,RT-PCR检测候选基因及脂质代谢相关基因表达含量。结果表明:Ber组卵母细胞颗粒细胞扩散率(P0.05)和第一极体排出率较对照组均差异显著(P0.01);相比于对照组和GV组间的差异miRNA,Ber组上调4个在卵母细胞成熟中差异表达的miRNA,下调1个miRNA;Ber组卵母细胞脂滴含量明显少于对照组;Ber组卵母细胞中与脂质代谢相关的基因的表达均极显著低于对照组(P0.01),且对照组卵母细胞极显著低于GV组(P0.01)。Ber可能通过影响miRNA及脂质代谢相关的基因,降低猪卵母细胞中脂滴的含量,进而促进猪卵母细胞体外成熟。
[Abstract]:The purpose of this experiment was to explore the effect of Berberine (Ber) on the in vitro maturation of porcine oocytes and the effects on the expression difference of miRNA and the genes related to lipid droplet metabolism. The method was used to obtain the cumulus oocyte complex, the 0.1% hyaluronidase removed the immature oocytes from the GV group, and the TCM-199 culture medium was used as the extracorporeal liquid. As the control group, Ber was added to group Ber to observe the maturation effect of oocyte. Gene chip technique was used to analyze the difference of miRNA in the process of porcine oocyte maturation in vitro, the change of lipid droplet content by Nile red staining, the candidate gene of RT-PCR and the expression content of lipid metabolism related genes. The results showed that the Ber group was the egg of Ber. The diffusion rate (P0.05) and the discharge rate of the first polar body were significantly different in the mother cell (P0.01). Compared to the control group and the GV group, the difference was miRNA, and the Ber group up regulated 4 differentially expressed miRNA and 1 miRNA in oocyte maturation, and the oocyte lipid droplet content in Ber group was significantly less than that in the control group; and in the Ber group of oocytes and lipid, the lipid content was significantly lower than that in the control group. The expression of metabolic related genes was significantly lower than that of the control group (P0.01), and the control group was significantly lower than the GV group (P0.01).Ber (P0.01), which may reduce the lipid droplet content in porcine oocytes by affecting the genes related to miRNA and lipid metabolism, and thus promote the maturation of porcine oocytes in vitro.
【作者单位】：北京农学院动物科学技术学院;
【基金】：国家自然基金项目(31572402) 科技创新服务能力建设-科研计划(市级)重点项目(KZ201610020017)
【分类号】：S828.3

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