不同成熟期桃品种NAC基因遗传多样性研究

发布时间：2018-05-30 09:23

本文选题：桃 + NAC转录因子　；参考：《江苏农业科学》2017年22期

【摘要】：从11个桃品种嫩叶中分别提取基因组DNA,根据GDR数据库中NAC基因序列信息,设计特异引物,PCR扩增,对产物克隆测序。然后,使用DNAman软件进行核苷酸和氨基酸序列比对,使用ORF Finder获得开放阅读框和推导氨基酸序列,使用CDD工具进行蛋白质结构域分析,利用Blastp工具搜索同源蛋白,采用Mega软件绘制系统进化树。结果发现,NAC蛋白第16~140位存在1个NAC结构域,N端比较保守,C端则多样性比较高;核苷酸变异有SNP和Indel 2类,津柳早红和小白桃在第3外显子区SNP和Indel变异数量非常多,说明这2个品种的NAC基因结构具有独特性。蛋白比对发现,桃NAC与梅同源性最高,其次是鸭梨;进化树体现了物种的亲缘关系。结果表明,参试品种NAC基因存在比较丰富的遗传多样性,在保守区内的氨基酸变异可能影响蛋白的功能。
[Abstract]:Genomic DNAs were extracted from tender leaves of 11 peach cultivars. According to the sequence information of NAC gene in GDR database, specific primers were designed for PCR amplification, and the products were cloned and sequenced. Then, nucleotide and amino acid sequence alignment was carried out with DNAman software, open reading frame and deduced amino acid sequence were obtained by ORF Finder, protein domain was analyzed by CDD tool, homologous protein was searched by Blastp tool. Mega software is used to draw the phylogenetic tree. The results showed that the N-terminal diversity of the N-terminal of one NAC domain was relatively high, and the nucleotide mutations were SNP and Indel 2, and the number of SNP and Indel mutations in exon 3 of Jinliusaonghong and Xiaobai peach were very large. The results showed that the NAC gene structure of the two cultivars was unique. Protein comparison showed that peach NAC had the highest homology with plum, followed by Yali pear, and the phylogenetic tree reflected the phylogenetic relationship of the species. The results showed that there was abundant genetic diversity in the NAC gene, and the amino acid variation in the conserved region might affect the function of the protein.
【作者单位】：天津农学院园艺园林学院;
【基金】：天津市应用基础与前沿技术研究计划一般项目(编号:14JCYBJC30100) 天津市科技型中小企业技术创新资金(编号:14ZXCXNC00063) 天津市高等学校科技发展基金(编号:20130617) 国家大学生创新创业训练计划(编号:201410061007) 天津市2014年度“131”创新型人才培养工程天津市高校第二批“优秀青年教师资助计划”
【分类号】：S662.1

【相似文献】