百脉根4个共生基因在水稻中的共表达及其对水稻转录组的影响

发布时间：2018-05-30 10:28

本文选题：百脉根 + 根瘤共生　；参考：《华中农业大学学报》2017年04期

【摘要】：为建立水稻-根瘤菌共生固氮体系,将百脉根共生信号转导途径中的4个功能基因(LHK1、NSP1、NSP2和NIN)转入水稻,获得稳定转化的转基因水稻,并初步检测转基因水稻的表型和转录组水平上的差异。根瘤菌感染试验结果显示,根瘤菌侵染对照(转入空载体)和转基因水稻的根毛和表皮的比率没有显著变化,但接种根瘤菌14d后,对照与转基因水稻的侧根原基数有显著差异,转共生基因水稻的侧根原基明显增多。利用水稻cDNA芯片,检测转基因水稻组与对照组在接种根瘤菌7d和不接种根瘤菌条件下根的转录组变化,结果显示,转基因组与对照组水稻在植物防御反应、激素信号转导途径相关基因均有差异性表达,表明豆科共生基因影响了水稻对根瘤菌的反应。
[Abstract]:In order to establish a symbiotic nitrogen fixation system of rice-rhizobium, four functional genes, LHK1, NSP1, NSP2 and NIN, were transferred into rice to obtain stable transformed transgenic rice. The phenotypic and transcriptional differences of transgenic rice were preliminarily detected. The results of Rhizobium infection test showed that the ratio of root hair and epidermis of Rhizobium infection control and transgenic rice did not change significantly, but after inoculation of Rhizobium for 14 days, there was significant difference between control and transgenic rice in lateral root primordial number. The lateral root primordium of transgenic rice increased significantly. The transcriptional changes of rice roots in transgenic rice group and control group under inoculation of rhizobia for 7 days and without rhizobia inoculation were detected by cDNA microarray. The results showed that transgenic rice and control group were responding to plant defense. The differentially expressed genes related to hormone signal transduction pathway indicated that the symbiotic genes of legumes affected the response of rice to rhizobia.
【作者单位】：农业微生物学国家重点实验室/华中农业大学生命科学技术学院;
【基金】：国家重点基础研究发展计划“973”项目(2010C126502) 高等学校博士学科点专项(20130146130003)
【分类号】：S511

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