日本鳗鲡TLR21基因的鉴定、免疫应答与启动子分析

发布时间：2018-05-30 11:17

本文选题：日本鳗鲡 + TLR　；参考：《水生生物学报》2017年02期

【摘要】：为研究TLR21(Toll like receptor 21)在低等脊椎动物中的功能及表达调控机制,我们扩增获得了日本鳗鲡TLR21(Aj TLR21)c DNA序列,其编码的蛋白具有TLR家族的共同特征。Aj TLR21基因结构与其他鱼类和两栖类TLR21相同,由单个外显子编码。荧光定量结果显示,Aj TLR21在血液、鳃、脾脏、中肾等11个组织/器官中转录表达,其中在血液中表达量最高。经Poly I:C诱导后8h,Aj TLR21在脾脏和中肾中的表达量显著性上调;诱导后16h,Aj TLR21在血液、鳃、肠和脾脏中的表达量显著性上调(P0.05)。双荧光素酶报告基因结果显示,在Aj TLR21 5′上游调控序列 1179 bp到+117 bp存在Poly I:C调节的正调控元件。经Edwardsiella tarda诱导后16h和72h,Aj TLR21分别在血液和中肾组织的表达量显著性上调,表明Aj TLR21同时也参与了抗细菌免疫应答,其在机体免疫系统中的功能具有多样性。研究对于理解日本鳗鲡Aj TLR21的免疫学功能具有重要的理论意义和应用价值。
[Abstract]:In order to study the function and expression regulation mechanism of TLR21(Toll like receptor 21 in low vertebrate, we amplified the TLR21(Aj TLR21)c DNA sequence of Japanese eel. The protein encoded by Aj TLR21 has the same structure as other fish and amphibian TLR21, and is encoded by a single exon. Fluorescence quantitative analysis showed that Aj TLR21 was transcribed in 11 tissues / organs such as blood, Gill, spleen and mesonephrosis, and the highest expression was found in blood. The expression of Aj TLR21 was significantly up-regulated in spleen and midkidney at 8 h after induction by Poly I: C, and significantly up-regulated in blood, Gill, intestine and spleen at 16 h after induction. The results of double luciferase reporter gene showed that there were positive regulatory elements of Poly I: C in the upstream regulatory sequence of Aj TLR21 5'from 1179 BP to 117bp. The expression of Aj TLR21 was significantly up-regulated in blood and midkidney tissues at 16 h and 72 h after induction by Edwardsiella tarda, indicating that Aj TLR21 also participated in the anti-bacterial immune response, and its function in the immune system was diverse. The study has important theoretical significance and application value for understanding the immunological function of Japanese eel Aj TLR21.
【作者单位】：集美大学水产学院;鳗鲡现代产业技术教育部工程研究中心;中国科学院水生生物研究所淡水生态与生物技术国家重点实验室;福建省海洋生物资源开发利用协同创新中心;
【基金】：福建省自然科学基金(2014J05042、2012J06008) 国家自然科学基金(31402329、31174238和U1205123)资助~~
【分类号】：S917.4

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