隐丹参酮对表皮葡萄球菌生物膜形成关键调控基因表达的抑制作用

发布时间：2018-05-30 23:23

本文选题：表皮葡萄球菌 + 生物膜　；参考：《广东医学》2017年S1期

【摘要】：目的探讨隐丹参酮对表皮葡萄球菌(SE)生物膜形成中关键调控基因的抑制作用。方法体外建立SE黏附、聚集和成熟各阶段生物膜模型,采用RT-PCR检测在隐丹参酮作用下;SE生物膜形成中关键调控基因ica A、atl E、aap、lux S的表达变化。结果 28μg/m L的隐丹参酮能明显抑制生物膜形成中黏附、聚集、成熟整个过程4个关键调控基因的表达,差异有统计学意义(P0.05);32μg/m L的隐丹参酮对黏附和聚集阶段生物膜形成中关键调控基因lux S有抑制作用,差异有统计学意义(P0.05),能使ica A、atl E、aap基因表达量下降,但差异无统计学意义(P0.05),增加成熟阶段生物膜形成中4个关键基因的表达量,但差异无统计学意义(P0.05);此外,128μg/m L的隐丹参酮与32μg/m L的隐丹参酮相比,前者生物膜形成中4个关键基因的表达明显低于后者,差异有统计学意义(P0.05)。结论隐丹参酮对SE各阶段生物膜形成中关键调控基因ica A、atl E、aap、lux S表达具有抑制作用,且这种抑制作用存在一定的剂量和时间效应。
[Abstract]:Objective to investigate the inhibitory effect of cryptotanshinone on the key regulatory genes in the biofilm formation of Staphylococcus epidermidis (SEE). Methods SE adhesion, aggregation and maturation biofilm models were established in vitro, and the expression of ica Agnatl Eaaplux S, a key regulatory gene in SE biofilm formation under the action of cryptotanshinone, was detected by RT-PCR. Results Cryptotanshinone 28 渭 g / mL could significantly inhibit the expression of four key regulatory genes in the formation of biofilm, such as adhesion, aggregation and maturation. The difference was statistically significant that cryptotanshinone (32 渭 g / mL) inhibited the expression of lux S, a key regulatory gene in biofilm formation during adhesion and aggregation. The difference was statistically significant (P 0.05), and could decrease the expression of ica Atoatl Eaap gene. However, there was no significant difference in the expression of four key genes in mature biofilm formation, but the difference was not statistically significant. In addition, there was no significant difference between cryptotanshinone (128 渭 g / mL) and cryptotanshinone (32 渭 g / mL) in mature biofilm formation. The expression of four key genes in biofilm formation of the former was significantly lower than that of the latter, and the difference was statistically significant (P 0.05). Conclusion Cryptotanshinone has an inhibitory effect on the expression of the key regulatory gene ica Atoatl Eaplux S in the various stages of SE biofilm formation, and this inhibition has a dose and time effect.
【作者单位】：成都中医药大学医学技术学院;成都市温江区人民医院检验科;
【基金】：四川省科技计划项目(编号:2015JY0159)
【分类号】：R285

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