贵州地方山羊FSHR基因与繁殖性状的相关性研究

发布时间：2018-05-31 00:24

本文选题：贵州地方山羊 + 促卵泡素受体基因(FSHR)　；参考：《农业生物技术学报》2017年01期

【摘要】：促卵泡素受体(follicle stimulating hormone receptor,FSHR)参与卵泡的生长发育和分化成熟,与动物的繁殖性状存在一定的关联性。为了探究FSHR基因多态性及其m RNA的表达量与黔北麻羊(Capra hircus)繁殖性状之间的关系,本研究选取高繁殖力的黔北麻羊和低繁殖力的贵州黑山羊(Capra hircus)为实验对象,采用聚合酶链式反应-单链构象多态(polymerase chain reaction-single strand conformation polymorphism,PCR-SSCP)和PCR技术对2个品种FSHR基因第1和10外显子的多态性进行检测,并分析突变位点与产羔数之间的相关性。结果显示,2个实验群体在第1和10外显子各存在1个SNP位点,即C126T和C1246A。在黔北麻羊中,BB型个体的产羔数显著高于AA型和AB型(P0.05);在贵州黑山羊中,2个SNPs位点不同基因型个体之间的产羔数均差异不显著(P0.05)。此外,q RT-PCR结果显示,FSHR基因在BB型黔北麻羊各组织的表达量均高于AA型,且在BB型黔北麻羊发情间期下丘脑组织和发情期卵巢组织的表达量极显著高于AA型黔北麻羊(P0.01),在发情间期和发情期的子宫组织中FSHR基因表达量在两种基因型的个体间也达到显著差异水平(P0.05)。结果提示,FSHR基因对于调控黔北麻羊的多羔性状具有重要作用。研究结果为从分子水平探索影响黔北麻羊繁殖性能的候选基因,以及建立高繁殖力的贵州地方山羊核心群提供基础资料。
[Abstract]:The follicle stimulating hormone receptor (follicle stimulating hormone receptor, FSHR) participates in the growth and differentiation of follicles and has a certain correlation with the reproductive traits of the animals. In order to explore the relationship between the polymorphism of the FSHR gene and the expression of M RNA and the reproductive traits of the northern Guizhou sheep (Capra hircus), this study selects the high fecundity of Guizhou. North Ma sheep and low fecundity Guizhou black goat (Capra hircus) were tested by polymerase chain reaction single strand conformation polymorphism (polymerase chain reaction-single strand conformation polymorphism, PCR-SSCP) and PCR techniques to detect the polymorphism of first and tenth exons of 2 FSHR genes, and the mutation sites and production were analyzed. The correlation between the number of lambs showed that there were 1 SNP loci in the first and tenth exons of the 2 experimental groups, that is, C126T and C1246A. in the northern Guizhou sheep, the number of lambing of BB type individuals was significantly higher than that of AA and AB (P0.05). In Guizhou black goats, there was no significant difference in the number of lambs among the 2 SNPs loci in Guizhou black goats. Moreover, The results of Q RT-PCR showed that the expression of FSHR gene in all the tissues of BB sheep in northern Guizhou was higher than that of the AA type, and the expression of the hypothalamus and the oestrus of the oestrus was significantly higher than that of the AA type Qian North Ma sheep (P0.01). The expression of the FSHR gene in the uterine tissues of the estrus and estrus period was two genotypes in the oestrus and estrus period. The results suggest that the FSHR gene plays an important role in the regulation of the multi lamb traits in the North Guizhou Ma sheep. The results provide the basic information for exploring the candidate genes affecting the reproductive performance of the North Guizhou sheep from the molecular level, as well as the establishment of the high fecundity Guizhou local goat core group.
【作者单位】：贵州大学高原山地动物遗传育种与繁殖省部共建教育部重点实验室;贵州大学动物科学学院/贵州省动物遗传育种与繁殖重点实验室;蒙阴县畜牧兽医局;贵州省种畜禽种质测定中心;
【基金】：贵州省科技重大专项(黔科合重大专项字(2013)6008)
【分类号】：S827

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