高强度光照损伤致TgAPPswePS1转基因鼠脉络膜新生血管形成

发布时间：2018-05-31 00:39

本文选题：光照损伤 + 转基因鼠　；参考：《眼科新进展》2017年02期

【摘要】：目的研究在光照损伤作用下TgA PPsweP S1转基因鼠脉络膜新生血管形成情况。方法以20只6个月龄阿尔茨海默病转基因鼠(TgA PPsweP S1鼠)为研究对象,其中12只行10 000 lux光照处理,每天光照12 h、连续光照6个月为实验组,余8只不作处理为对照组,实验组与对照组小鼠在实验结束时进行取材,切片行HE染色及甲苯胺蓝染色观察视网膜各层结构改变,测量新生血管形成数量,同时检测实验组与对照组视网膜色素上皮(retinal pigment epithelium,RPE)铺片上β-淀粉样蛋白(β-amyloid protein,Aβ)与血管内皮生长因子表达情况,予以定量并进行统计学分析。结果与对照组比较,光照6个月后,实验组小鼠视网膜发生明显改变,其中以外核层与光感受器层变化最明显;RPE细胞出现空泡、色素不均一、细胞间的连接断裂等改变,视网膜切片上可见脉络膜新生血管突破RPE进入视网膜内,对新生血管进行统计,发现脉络膜新生血管发生率为18.75%。光照损伤后,血管内皮生长因子在实验组RPE上呈阳性表达,可见细胞浆内弥散性表达和沿血管壁的阳性染色,较对照组表达量增加(6.59±1.14)倍,差异有统计学意义(P0.05)。Aβ在实验组的RPE铺片上存在强阳性表达,通过Z-stack检测发现Aβ沉积类似Drusen状,位于RPE下;而对照组中未见此类免疫阳性反应,与对照组比较Aβ沉积增加(6.45±2.93)倍,差异存在统计学意义(P0.05)。结论强光光照损伤TgA PPsweP S1鼠后,视网膜存在明显变化,可发生脉络膜新生血管,提示阿尔茨海默病转基因鼠在光照损伤下可以作为脉络膜新生血管的一种动物研究模型。
[Abstract]:Objective to study the choroidal neovascularization in TgA PPsweP S1 transgenic mice induced by light damage. Methods Twenty 6-month-old transgenic mice with Alzheimer's disease (TGA PPsweP S1) were studied. Twelve of them were treated with 10 000 lux light for 12 hours a day for 6 consecutive months as experimental group and the remaining 8 as control group. At the end of the experiment, the mice in the experimental group and the control group were selected, the sections were stained with HE and toluidine blue staining to observe the structural changes of each layer of the retina, and the number of neovascularization was measured. At the same time, the expression of 尾 -amyloid protein A 尾 and vascular endothelial growth factor (VEGF) on retinal pigment epithelium (RPE) of experimental group and control group were detected, and the expression of 尾 -amyloid protein A 尾 and vascular endothelial growth factor (VEGF) were quantitatively and statistically analyzed. Results compared with the control group, there were obvious changes in retina of the experimental group after 6 months of illumination, in which the outer nuclear layer and the photoreceptor layer had the most obvious changes, such as vacuole, pigment heterogeneity, intercellular junction breakage and so on. Choroidal neovascularization broke into the retina through RPE on retinal sections. The incidence of choroidal neovascularization was 18.75. The positive expression of vascular endothelial growth factor (VEGF) on RPE was observed in the experimental group after irradiation. The expression of VEGF in the cytoplasm and the positive staining along the vascular wall was 6.59 卤1.14 times higher than that in the control group. There was a strong positive expression of P0.05A 尾 on RPE in the experimental group. The positive expression of A 尾 was found to be similar to that of Drusen in the experimental group by Z-stack detection, but no such immunopositive reaction was found in the control group, and the A 尾 deposition increased 6.45 卤2.93 times compared with the control group, and the positive expression of A 尾 in the control group was 6.45 卤2.93 times higher than that in the control group. The difference was statistically significant (P 0.05). Conclusion there are obvious changes in the retina and choroidal neovascularization in TgA PPsweP S1 rats after intense light irradiation, which suggests that transgenic mice with Alzheimer's disease can be used as an animal model of choroidal neovascularization under illumination injury.
【作者单位】：温州医科大学附属第二医院眼科;西安市第四医院眼科;广东医科大学衰老研究所东莞校区;中山大学中山眼科中心眼科学国家重点实验室;
【基金】：国家自然科学基金资助(编号:81400424) 陕西省科学技术研究发展计划项目(编号:2014K11-03-07-04) 温州市科技计划项目(编号:Y20140140)~~
【分类号】：R749.16;R77

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