芒果果实转录组数据组装及基因功能注释

发布时间：2018-05-31 02:22

本文选题：芒果 + 转录组　；参考：《热带作物学报》2016年11期

【摘要】：以红芒6号为试材,对生长发育各时期果皮与果肉样品提取RNA后等量混合进行转录组测序,共获得了68 419 722个reads,包含6 157 744 980个核苷酸序列信息,平均读长90 bp,序列信息全部登陆NCBI数据库(SRP035450)。对reads进行拼接,共获得124 002个Contig片段,进一步组装获得54 207个Unigene片段,平均长度为838 bp,阈值小于10-5的序列中共有42 515(78.43%)个unigenes被注释到公共蛋白数据库。其中,35 198个被注释到生物学过程、分子功能和细胞组分GO类别的44个功能组,14 619个Unigene匹配到25类COG功能组,23 741个Unigene被富集到128个KEGG代谢通路,包括代谢途径、次生代谢的生物合成、植物-病原物互作、植物激素信号转导、苯丙氨酸生物合成、淀粉和蔗糖代谢、类黄酮类化合物合成等。本研究将为进一步的芒果功能基因克隆、基因表达分析、分子标记开发等研究奠定基础。
[Abstract]:Using Hongmang 6 as a sample, the RNA from pericarp and pulp samples were extracted and sequenced. A total of 68 419 722 readswere obtained, including 6 157 744,980 nucleotide sequence information. The average read length is 90 BP, and all the sequence information is recorded in NCBI database (SRP 035450). A total of 124,002 Contig fragments were obtained from reads splicing, and 54,207 Unigene fragments were further assembled, with an average length of 838 BP. A total of 42 515 unigenes were annotated into the common protein database in the sequence with a threshold of less than 10-5. 35,198 of them were annotated to biological process, 14,619 Unigene of 44 functional groups of molecular function and cell components were matched to 23,741 Unigene of 25 COG functional groups, and 23 741 Unigene were enriched to 128 KEGG metabolic pathways, including metabolic pathways. Biosynthesis of secondary metabolism, plant-pathogen interaction, plant hormone signal transduction, phenylalanine biosynthesis, starch and sucrose metabolism, flavonoid synthesis, etc. This study will lay a foundation for the further research of mango functional gene cloning, gene expression analysis and molecular marker development.
【作者单位】：中国热带农业科学院南亚热带作物研究所农业部热带果树生物学重点实验室;
【基金】：农业行业科研专项子项目(No.201203092-3) 广东省自然科学基金-粤西北创新人才专项(No.2016A030307006)
【分类号】：S667.7

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