杂交杨转录因子Pdt-ERF3抗锈病基因表达

发布时间：2018-05-31 08:51

本文选题：转录调控因子 + ERF　；参考：《森林工程》2016年06期

【摘要】：落叶松-杨栅锈菌(Melampsora larici-populina)严重危害多种杨树。为探究转录调控因子ERF3在杂交杨抗锈菌反应中的分子机理,克隆到弱感病性树种杂交杨(Populus deltoides×P.trichocarpa)和感病树种欧美杨(Populus×euramericana)ERF3基因:Pdt-ERF3和Pnd-ERF3。采用荧光定量Q-PCR技术对接种E4强致病性落叶松-杨栅锈菌前后PdtERF3和Pnd-ERF3基因表达量变化进行分析。落叶松-杨栅锈菌接种后,Pdt-ERF3表达量在12 hpi达到高峰期,PndERF3表达量在24 hpi显著上调,在96 hpi达到高峰期。Pnd-ERF3比Pdt-ERF3表达量高峰期推迟84 h。除168 hpi时Pnd-ERF3与Pdt-ERF3表达均有下降外,2个基因表达量在锈菌侵染的多个阶段均存在差异。根据拟南芥(Arabidopsis thaliana)同源基因预测Pdt-ERF3受丝裂原活化蛋白激酶MAPK6调控。本研究为进一步系统研究杨树ERF基因与抗性反应的调控机理及杨树抗病育种工作提供理论依据。
[Abstract]:Melampsora larici-populinina (Larix gmelini) is a serious hazard to many poplars. In order to explore the molecular mechanism of transcription regulator ERF3 in the resistance to rust of hybrid poplar, Populus deltoides 脳 P. trichocarpa, a weakly susceptible species, and a susceptible tree species, Populus 脳 euramericana)ERF3, were cloned into poplar, Pdt-ERF3 and Pnd-ERF3. Fluorescence quantitative Q-PCR technique was used to analyze the changes of PdtERF3 and Pnd-ERF3 gene expression before and after inoculation with E4 strong pathogenic larch-poplar rust. After inoculation, Pdt-ERF3 expression reached its peak at 12 hpi and PndERF3 increased significantly at 24 hpi, and reached the peak at 96 hpi. Pnd-ERF3 was 84 hours later than the peak of Pdt-ERF3 expression. Except for the decrease of Pnd-ERF3 and Pdt-ERF3 expression at 168 hpi, there were differences between the two genes in many stages of rust infection. The homologous gene Arabidopsis thaliana predicted that Pdt-ERF3 was regulated by mitogen-activated protein kinase (MAPK6). This study provides a theoretical basis for further systematic study on the regulation mechanism of ERF gene and resistance response of poplar and the breeding of poplar disease resistance.
【作者单位】：东北林业大学林学院;
【基金】：国家自然科学基金项目(31500524) 中央高校基本科研业务费专项资金项目(2572015EY02,2572015CA09,2572016CA04) 大学生创新性实验计划项目(201510225129)
【分类号】：S763.7

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