沉默HMGB1基因对非小细胞肺癌细胞上皮间质转化的影响及作用机制

发布时间：2018-05-31 18:59

本文选题：非小细胞肺癌 + 高迁移率族蛋白　；参考：《山东医药》2017年36期

【摘要】：目的探讨沉默高迁移率族蛋白1(HMGB1)基因对非小细胞肺癌细胞上皮间质转化(EMT)的影响及其作用机制。方法取传3代非小细胞肺癌A549细胞,随机分为si-HMGB1组、阴性对照组、空白组。si-HMGB1组、阴性对照组分别转染HMGB1 siRNA序列、阴性对照siRNA序列,空白组不予转染。各组继续孵育72 h,检测HMGB1 mRNA表达。收集各组孵育72 h细胞,采用Western blotting法检测EMT相关蛋白(E-cadherin、VDR、N-cadherin、Vimentin)和TGF-β/Smad信号通路相关蛋白(Snail、NF-κB p65、p-NF-κB p65)表达。结果 si-HMGB1组HMGB1 mRNA相对表达量明显低于阴性对照组和空白组(P均0.05),而阴性对照组与空白组比较P0.05。si-HMGB1组E-cadherin、VDR蛋白相对表达量明显高于阴性对照组和空白组(P均0.05),N-cadherin、Vimentin蛋白相对表达量明显低于阴性对照组和空白组(P均0.05),而阴性对照组与空白组各EMT相关蛋白相对表达量比较P均0.05。si-HMGB1组Snail、p-NF-κB p65蛋白相对表达量明显低于阴性对照组和空白组(P均0.05),而阴性对照组与空白组比较P均0.05。三组间NF-κB p65蛋白相对表达量比较P均0.05。结论沉默HMGB1基因能够逆转非小细胞肺癌细胞EMT,其机制可能与阻断Snail/NF-κB信号通路有关。
[Abstract]:Objective to investigate the effect of silencing high mobility group protein 1 (HMGB1) gene on epithelial mesenchymal transformation (EMTT) of non-small cell lung cancer (NSCLC) cells and its mechanism. Methods A549 cells were randomly divided into si-HMGB1 group, negative control group and blank group. The negative control group was transfected with HMGB1 siRNA sequence, the negative control group with siRNA sequence, and the blank group with no transfection. All groups were incubated for 72 hours to detect the expression of HMGB1 mRNA. The expression of E-cadherin (E-cadherin), N-cadherin (N-cadherin) and Snail- 尾 / Smad signal transduction related protein (Snail- 尾 / Smad) was detected by Western blotting assay. Results the relative expression of HMGB1 mRNA in si-HMGB1 group was significantly lower than that in negative control group and blank group (P 0.05). The relative expression of E-cadherin si-HMGB1 protein in P0.05.si-HMGB1 group was significantly higher than that in negative control group and blank group (P 0.05). The relative expression of EMT related protein in the negative control group and the blank group was significantly lower than that in the negative control group and the blank group, and the relative expression of Snail-p-NF- 魏 B p65 protein in the P 0.05.si-HMGB1 group was significantly lower than that in the negative control group and the blank group. The negative control group was significantly higher than the blank group (P < 0.05). The relative expression of NF- 魏 B p65 protein in the three groups was significantly higher than that in the control group (P < 0.05). Conclusion silencing HMGB1 gene can reverse EMTs in non-small cell lung cancer cells, which may be related to blocking Snail / NF- 魏 B signaling pathway.
【作者单位】：西南医科大学附属医院;
【基金】：基金项目:卫生部医药卫生科技发展研究(卫技中[2011]24号)
【分类号】：R734.2

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