绵羊FSHR基因可变剪接体的克

发布时间：2018-06-02 18:55

本文选题：绵羊 + FSHR基因　；参考：《江苏农业学报》2017年03期

【摘要】：根据Gen Bank中绵羊促卵泡激素受体基因(FSHR)的mRNA序列(登录号:NM_001009289.1)设计引物,反转录PCR(RT-PCR)扩增蛋白编码区(CDs)区,以获取绵羊FSHR基因可变剪接体,并通过Western blot验证其在组织中的表达,通过免疫组织化学方法检测其在卵泡中的表达位置。结果表明,在绵羊卵巢中除了全长的、编码695个氨基酸的FSHR表达产物外,还鉴定到分别编码694个、633个、595个、582个和533个氨基酸的FSHR可变剪接形式的转录产物。在卵泡中主要以695(694)、633和595 3种转录本形式存在。但在颗粒细胞中绵羊促卵泡激素受体主要以经典分子即695个氨基酸的形式存在。在卵泡发育过程中,FSHR蛋白质含量发生变化:在初级卵泡中,FSHR主要在卵母细胞中表达,而不是在单层的颗粒细胞中表达,在次级卵泡中,FSHR在颗粒细胞和卵母细胞中均不表达,到三级卵泡(有腔卵泡)时,FSHR蛋白质在颗粒细胞中表达丰富。可见,绵羊FSHR基因虽然存在可变剪接体的结构,但其功能主要取决于经典型的695个氨基酸蛋白质分子,并且对初级卵泡的发育具有一定的作用。
[Abstract]:According to the mRNA sequence of sheep follicle stimulating hormone receptor gene (FSHR) in Gen Bank (accession number: NMash009289.1), primers were designed to amplify the protein coding region (CDss) by reverse transcription PCRRT-PCR (reverse transcription PCRRT-PCR) to obtain the variable splicing of sheep FSHR gene, and its expression in tissues was verified by Western blot. Immunohistochemical method was used to detect its expression in follicles. The results showed that in addition to the full-length FSHR expression products encoding 695 amino acids in ovaries of sheep, the mutable splicing transcripts of FSHR were also identified. In follicles, there are three transcription forms: 695, 694, 633 and 595, respectively. However, sheep follicle stimulating hormone receptor in granulosa cells mainly exists in the form of 695 amino acids. The protein content of FSHR changed during follicular development: in primary follicles, FSHR was expressed mainly in oocytes, not in monolayer granulosa cells, but in secondary follicles, FSHR was not expressed in granulosa cells and oocytes. FSHR protein was abundant in granulosa cells by the third stage follicle (with antral follicles). It can be seen that although sheep FSHR gene has a variable splice structure, its function mainly depends on the classical 695 amino acid protein molecules and plays a certain role in the development of primary follicles.
【作者单位】：新疆畜牧科学院生物技术研究所;
【基金】：新疆维吾尔自治区自然科学基金项目(2014211A068)
【分类号】：S826

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