海南五指山猪TLR2基因克隆及生物信息学分析

发布时间：2018-06-03 02:20

本文选题：海南 + 五指山猪　；参考：《黑龙江畜牧兽医》2017年17期

【摘要】：为了探讨海南五指山猪TLR2基因的分子结构特征及相关遗传变异,为研究TLR2基因遗传变异与免疫疾病的关系打下基础,试验采用PCR技术对该基因组序列进行克隆、测序及相关生物信息学分析。结果表明:获得2 649 bp的五指山猪TLR2基因的DNA序列,其基因编码区全长2 358 bp,编码785个氨基酸;与Gen Bank中发布的普通猪TLR2基因序列相比,共发现9处突变,其中在编码区序列存在2处突变,属于有义突变;与普通猪、马、牛、绵羊、人、黑猩猩、猕猴、犬、小鼠、鸡、斑马鱼的氨基酸序列的一致性分别为99.7%、82.1%、81.4%、80.6%、78.4%、78.4%、77.8%、77.3%、68.2%、50.7%、40.4%;五指山猪TLR2编码的蛋白主要位于质膜,属于分泌性蛋白、亲水性蛋白和跨膜蛋白,有信号肽和5个糖基化修饰位点,存在较多的丝氨酸(Ser)和苏氨酸(Thr)磷酸化位点;不同物种TLR2基因在进化过程中具有较高的保守性。说明TLR2基因可作为今后研究海南五指山猪免疫疾病的重要指标之一。
[Abstract]:In order to study the molecular structure and related genetic variation of TLR2 gene in Wuzhishan pig, Hainan, and to study the relationship between genetic variation of TLR2 gene and immune disease, the genomic sequence was cloned by PCR technique. Sequencing and bioinformatics analysis. The results showed that the DNA sequence of the TLR2 gene of Wuzhishan pig was 2 649 BP, and its coding region was 2 358 BP, encoding 785 amino acids, and 9 mutations were found compared with the normal TLR2 gene sequence published in Gen Bank. There are 2 mutations in the coding region, which belong to the sense mutation; and ordinary pigs, horses, cattle, sheep, human beings, chimpanzees, macaques, dogs, mice, chickens, The amino acid sequences of zebrafish were 99.72.1and 81.4, respectively. The amino acid sequences of zebrafish were 78.4 and 78.4, respectively. The protein encoded by Wuzhishan pig TLR2 was mainly located in the plasma membrane, which belonged to secreted protein, hydrophilic protein and transmembrane protein, and had signal peptides and five glycosylation modification sites. There were more serine and threonine Thr-phosphorylation sites, and TLR2 genes of different species were highly conserved in the course of evolution. The results suggest that TLR2 gene can be used as one of the important indexes for the future study of immune diseases in Wuzhishan pigs in Hainan.
【作者单位】：海南省农业科学院畜牧兽医研究所;海南省热带动物繁育与疫病研究重点实验室;
【基金】：国家自然科学基金项目(31560696) 海南省自然科学基金项目(20153080) 海南省农业科学院农业科技创新专项(CXZX201504)
【分类号】：Q78;S828

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