拟南芥开花诱导基因FT的蛋白表达及纯化

发布时间：2018-06-03 07:32

本文选题：成花素 + FT　；参考：《基因组学与应用生物学》2017年08期

【摘要】：FT蛋白是植物的成花素,在植物多条不同开花途径的交汇节点发挥重要作用。该蛋白在植物叶片中合成运输到顶端分生组织发挥作用。为了研究FT蛋白的运输机制,本研究利用原核表达技术体外诱导表达了His6-FT蛋白;利用His镍柱纯化获得了高质量的His6-FT融合蛋白;利用Western blotting免疫印迹技术确定该His6-FT蛋白能够被anti-His单克隆抗体特异识别。试验获得的正确融合的His6-FT蛋白为进一步研究FT蛋白的转运机制奠定了良好的基础。
[Abstract]:FT protein is the flowering hormone of plants and plays an important role in the intersecting nodes of many different flowering pathways in plants. The protein is synthesized and transported to the apical meristem in plant leaves. In order to study the transport mechanism of FT protein, the prokaryotic expression technique was used to induce the expression of His6-FT protein in vitro, and the high quality His6-FT fusion protein was obtained by His nickel column purification. Western blotting immunoblotting technique was used to determine that the His6-FT protein could be specifically recognized by anti-His monoclonal antibody. The correct fusion of His6-FT protein obtained from the experiment laid a good foundation for further study on the transport mechanism of FT protein.
【作者单位】：华北理工大学生命科学学院基因组学与计算生物学研究中心;
【基金】：国家自然科学基金(31401212) 河北省自然科学基金(C2014209134)共同资助
【分类号】：Q943.2

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