水稻OsCCCH基因家族的组织表达谱及胁迫诱导表达特征研究

发布时间：2018-06-03 23:43

  本文选题：CCCH锌指蛋白 + 非生物胁迫　； 参考：《分子植物育种》2016年09期

【摘要】：CCCH型锌指蛋白是一类生物体中广泛存在的转录因子,在多种植物生物学过程中起着关键的调控作用。在水稻全基因组中已发现67个CCCH基因,分为8个亚家族,而其中大部分基因的功能尚缺少研究。本研究通过荧光定量RT-PCR技术分析了水稻Os CCCH家族基因的组织表达模式以及盐、PEG、低温等非生物胁迫和脱落酸(ABA)对CCCH基因的诱导表达特征。结果表明,大部分Os CCCH基因在各组织中有不同的表达模式,约30%基因为低丰度表达,50%以上基因表现为中丰度表达,极少Os CCCH基因为高丰度表达,包括Os C3H2、Os C3H35、Os C3H41、Os C3H43、Os C3H58和Os C3H67这6个组织优势表达基因。多数Os CCCH基因都不同程度地受到盐、PEG、低温及ABA的诱导或抑制,表明这些CCCH锌指蛋白可能参与水稻的非生物胁迫响应;在四种胁迫下,有7个基因同时受到诱导,其中Os C3H17基因上调倍数高达17倍以上;有10个基因同时被抑制,其中Os C3H16基因下调倍数高达31倍以上。
[Abstract]:Zinc finger protein (CCCH) is a widespread transcription factor in a variety of organisms and plays a key role in many plant biological processes. 67 CCCH genes have been found in the whole rice genome, which are divided into 8 subfamilies, but the function of most of these genes has not been studied. In this study, the tissue expression patterns of rice Os CCCH family genes and the induced expression characteristics of CCCH gene under abiotic stress, such as low temperature and abscisic acid (Aba), were analyzed by fluorescence quantitative RT-PCR. The results showed that most of the Os CCCH genes had different expression patterns in different tissues, about 30% of the genes expressed in low abundance and over 50% of the genes expressed in medium abundance, and very few of the Os CCCH genes were expressed in high abundance. There are six dominant expression genes including Os C3H35OC3H41OC3H41OOC3H43OOs C3H58 and Os C3H67. Most of Os CCCH genes were induced or inhibited in different degrees by salt peg, low temperature and ABA, indicating that these CCCH zinc finger proteins may be involved in abiotic stress response of rice, and 7 genes were induced simultaneously under four stresses. The up-regulated multiple of Os C3H17 gene was more than 17 times, and 10 genes were inhibited at the same time, in which Os C3H16 gene down-regulated by more than 31 times.
【作者单位】： 重庆市农业科学院生物技术研究中心逆境农业研究重庆市重点实验室;
【基金】：重庆市农业科学院博士启动项目资助
【分类号】：Q943.2
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本文编号：1974778