中间锦鸡儿CiWRKY2基因克隆与表达分析

发布时间：2018-06-06 04:46

本文选题：CiWRKY + 中间锦鸡儿　；参考：《分子植物育种》2017年04期

【摘要】：通过RACE技术从中间锦鸡儿中克隆得到一个WRKY转录因子编码基因CiWRKY2,该基因g DNA为3 224 bp,含有4个内含子;cDNA长2 032 bp,开放阅读框1 725 bp,可以编码574个氨基酸,蛋白分子量大小约为63.29 kD,等电点为7.65。系统进化分析发现,CiWRKY2与豆科植物WRKY相似度最高,属于第一类WRKY蛋白。亚细胞定位显示CiWRKY2蛋白定位于细胞核。实时荧光定量结果表明CiWRKY2主要在叶、根中表达,并且该基因转录水平的表达受到干旱、盐碱、高温、低温和ABA诱导,表明CiWRKY2基因可能在植物抗逆中起到一定作用。
[Abstract]:A WRKY transcription factor encoding gene CiWRKY2 was cloned from Caragana intermedium by race technique. The gene's g DNA was 3 224 BP, containing 4 introns cDNA length of 2 032 BP, and open reading frame 1 725 BP, which could encode 574 amino acids. The molecular weight of the protein is about 63.29 KD and the isoelectric point is 7.65. Phylogenetic analysis showed that CiWRKY2 had the highest similarity with legume WRKY and belonged to the first kind of WRKY protein. Subcellular localization showed that CiWRKY2 protein was located in the nucleus. The results of real-time fluorescence quantitative analysis showed that CiWRKY2 was mainly expressed in leaves and roots, and the expression of CiWRKY2 was induced by drought, salinization, high temperature, low temperature and ABA, suggesting that CiWRKY2 gene might play a role in plant stress resistance.
【作者单位】：内蒙古农业大学生命科学学院;
【基金】：国家自然科学基金(31360169) 内蒙古产业创新创业人才团队内蒙古自治区科技创新团队(201503004)共同资助
【分类号】：Q943.2

【相似文献】