水杨酸及其信号转导基因表达在阳春砂果实发育过程中的变化

发布时间：2018-06-06 09:31

本文选题：阳春砂 + 果实发育　；参考：《广州中医药大学学报》2016年03期

【摘要】：【目的】对阳春砂发育过程中内源水杨酸含量及转导途径中的关键基因的关系进行深入分析。【方法】以6月、8月采集的阳春砂新鲜果实为实验材料,采用液相质谱联用(LC-MS/MS)的方法测定阳春砂种子团及果皮中水杨酸的含量并结合幼果期、成熟中期、成熟末期的阳春砂果实的高通量测序表达谱数据,分析3个发育时期中水杨酸信号转导通道中相关基因的表达模式。【结果】阳春砂果实发育过程中,种子团及果皮内水杨酸的含量变化一致,且与水杨酸信号转导途径的关键基因的表达量变化基本一致,均呈上升趋势。【结论】内源水杨酸与其信号转导基因之间存在密切的关系。推测为种子团内合成水杨酸后运输至果皮。
[Abstract]:[objective] to analyze the relationship between the content of endogenous salicylic acid and the key genes in transduction pathway during the development of Yangchun sand. [methods] fresh fruits collected in June and August were used as experimental materials. The content of salicylic acid in seeds and pericarp of yangchun sand was determined by liquid phase mass spectrometry (LC-MS / MS) and combined with high throughput sequencing expression data of young fruit stage, middle maturation stage and late mature stage. The expression patterns of related genes in the signal transduction pathway of salicylic acid in three developmental stages were analyzed. [results] the contents of salicylic acid in seeds and pericarp were the same during the development of yangchun sand fruit. The expression level of the key genes in the signal transduction pathway of salicylic acid is basically consistent with the change of the expression level, and the expression of these genes is on the rise. [conclusion] there is a close relationship between endogenous salicylic acid and its signal transduction genes. It was suggested that salicylic acid was synthesized in the seed and transported to the pericarp.
【作者单位】：广州中医药大学中药资源科学与工程研究中心;岭南中药资源教育部重点实验室;广州中医药大学中药学院;中国科学院昆明植物研究所资源植物与生物技术所级重点实验室;
【基金】：国家自然科学基金青年科学基金项目(编号:81303163) 广东省高等学校优秀青年教师培养计划项目(编号:Yq2013042) 广州中医药大学青年英才培养项目(编号:AAC414124A08)
【分类号】：S567.239

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