miR-15b靶基因预测、信号通路分析及靶基因鉴定

发布时间：2018-06-06 18:04

本文选题：miR-b + 生物信息学　；参考：《甘肃农业大学学报》2017年04期

【摘要】：【目的】对miR-15b靶基因进行预测、信号通路分析及鉴定,以期为miR-15b的功能研究奠定基础.【方法】利用Target Scan Release 7.0,PicTar和PITA数据库对miR-15b靶基因进行预测,再用DAVID数据库对预测出的靶基因集进行功能富集分析(gene ontology-analysis)及信号转导通路富集分析(KEGG pathway analysis).随后利用双荧光素酶报告试验对预测出miR-15b的靶基因丙酮酸脱氢酶激酶4(PDK4)和CD28进行验证.【结果】3个软件共同预测出miR-15b的靶基因有305个,其靶基因集合功能富集于蛋白激酶活性、GTP结合蛋白调控等分子功能,蛋白氨基酸磷酸化、细胞周期调控等生物学过程及微管相关复合体、转录因子复合体等细胞组分上.信号转导通路则显著富集于癌症相关信号通路.双荧光素酶报告实验结果显示CD28为miR-15b的靶基因,而PDK4不是miR-15b的靶基因.【结论】miR-15b在细胞增殖、凋亡和细胞周期调控等过程中发挥重要调控作用,且miR-15b通过调控CD28的表达对T细胞的受体激活发挥调控作用.
[Abstract]:[objective] to predict miR-15b target gene, signal pathway analysis and identification, so as to lay a foundation for the functional study of miR-15b. [methods] Target scan release 7.0 PicTar and PITA database were used to predict miR-15b target gene. Then, the predicted target gene sets were analyzed by functional enrichment analysis and signal transduction pathway enrichment analysis by DAVID database. Then the target genes of miR-15b were verified by double-luciferase report test, including pyruvate dehydrogenase kinase 4 PDK4) and CD28. [results] 305 target genes of miR-15b were predicted by three softwares. Its target gene set function is enriched in the molecular function of protein kinase activity and GTP binding protein regulation, protein amino acid phosphorylation, cell cycle regulation, microtubule-related complex, transcription factor complex and other cellular components. The signal transduction pathway was significantly enriched in the cancer-related signaling pathway. The results of double luciferase report show that CD28 is the target gene of miR-15b, while PDK4 is not the target gene of miR-15b. [conclusion] miR-15b plays an important role in cell proliferation, apoptosis and cell cycle regulation. MiR-15b regulates the receptor activation of T cells by regulating the expression of CD28.
【作者单位】：甘肃农业大学动物科学技术学院;重庆市畜牧科学院;甘肃省现代养猪工程技术研究中心;
【基金】：重庆市基本科研业务费项目(2013cstc-jbky-00105-zj,14402) 国家自然基金项目(31372284,31601930) 甘肃省科技重大专项项目“高繁殖力猪新品系选育研究”(092NKDA036)
【分类号】：Q78

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