茅苍术内参基因筛选及其在活性成分生物合成研究中的应用

发布时间：2018-06-07 05:09

本文选题：茅苍术 + 实时荧光定量PCR　；参考：《植物生理学报》2017年09期

【摘要】：本研究选取茅苍术转录组数据库中18S rRNA、β-Actin、EF-1α、GAPDH、UBQ1和UBQ2作为候选内参基因,运用实时荧光定量PCR技术分析6种候选内参基因在正常和干旱胁迫条件下不同组织中的表达,并用Delta CT、geNorm、Norm-Finder、Best Keeper及RefFinder等方法评价候选内参基因的表达稳定性。结果表明,UBQ2和EF-1α在正常条件下不同组织中的表达均较稳定,在干旱胁迫下EF-1α的表达最稳定。在此基础上,以UBQ2作为内参基因,研究正常条件下茅苍术活性成分生物合成关键酶HMGR、FPPS基因在其开花初期不同组织中的表达特性,结果显示花中HMGR、FPPS基因相对表达量均最高,根茎次之,茎和叶中相对表达量均较低。
[Abstract]:In this study, 18s rRNAs, 尾 -Actinine EF-1 伪 GAPDHU UBQ1 and UBQ2 were selected as candidate internal reference genes in the transcriptome database of Atractylodes macrocephala, and the expression of six candidate internal reference genes in different tissues under normal and drought stress was analyzed by real-time fluorescence quantitative PCR. The stability of candidate internal reference gene expression was evaluated by Delta CTG Norm Norm Findern Best Keeper and RefFinder. The results showed that the expression of UBQ2 and EF-1 伪 was stable in different tissues under normal condition, and the expression of EF-1 伪 was the most stable under drought stress. On the basis of this, using UBQ2 as internal reference gene, the expression characteristics of HMGRG FPPS gene, a key biosynthesis enzyme of Rhizoma Atractylodes, were studied under normal conditions. The results showed that the relative expression of HMGRG FPPS gene was the highest in different tissues in the early flowering stage of Rhizoma Atractylodes. The relative expression in stem and leaf was lower than that in rhizome.
【作者单位】：南京中医药大学药学院;
【基金】：国家自然科学基金(81573520) 江苏省中药资源产业化过程协同创新中心(ZDXM-3-24) 江苏省中药优势学科II期建设(ysxk-2014) “六大人才高峰”高层次人才项目(2012-YY-009) 江苏省“333高层次人才培养工程”~~
【分类号】：Q943.2;S567.239

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