小麦全基因组NBS类R基因分析及2AL染色体NBS-SSR特异标记开发

发布时间：2018-06-07 19:37

  本文选题：小麦 + 抗病　； 参考：《作物学报》2016年06期

【摘要】：NBS(nucleotide binding site)类基因是植物界中最重要的一类抗病基因。用信息学方法从普通小麦(Triticum aestivum L.)全基因组中分离出2406条含有NBS结构的完整蛋白序列,每条包含48~2272个氨基酸残基。根据NBS结构域两端是否连接CC或LRR结构域,将TaNBS分为N、CN、NL和CNL 4类。对TaNBS所在scaffold序列的SSR位点进行诊断,从1203条scaffold序列上发现2177个SSR位点,以二碱基重复位点最多,占73.5%。针对小麦2AL染色体上的51个SSR位点开发标记,缺体 四体和双端体验证结果表明,有39个标记(76.5%)为2AL特异标记,其中24个特异标记在抗白粉病材料Khapli(2AL上携带Pm4a)和感病材料Chancellor间存在多态性。利用近等基因系Khapli/8*Cc筛选出3个可能与Pm4a连锁的NBS-SSR标记,分别是Sxaas_2AL22、Sxaas_2AL39和Sxaas_2AL46。本研究开发的与抗病序列紧密连锁的特异SSR标记可用于2AL染色体上抗病新基因的检测以及已有抗病基因的候选序列筛选。
[Abstract]:Nucleotide binding site- like genes are the most important resistance genes in plant. Study on Triticum aestivum L. from Triticum aestivum L. by means of informatics 2406 complete protein sequences containing NBS structure were isolated from the whole genome, each containing 48 ~ 2272 amino acid residues. According to whether the two ends of the NBS domain are connected with CC or LRR domain, TaNBS is classified into NNCNNNL and CNL4. The scaffold loci of TaNBS were diagnosed and 2177 SSR loci were found from 1203 scaffold sequences. The most of them were two base repeats (73.5%). According to 51 SSR loci on wheat 2AL chromosome, the results of tetralogy and biterminal experience showed that 39 markers (76. 5) were 2AL specific markers. Among them, 24 specific markers carried Pm4a on the powdery mildew resistant material Khaplizao 2AL) and there was polymorphism between the susceptible material Chancellor and the anti-powdery mildew resistant material Khapliao 2AL. Three NBS-SSR markers, Sxaas2AL22, Sxaas2AL39 and Sxaas2AL46, which may be linked to Pm4a, were screened by using the near isogenic line Khapli-8Cc. The specific SSR markers closely linked to disease-resistant sequences can be used to detect new resistant genes on chromosome 2AL and to screen candidate sequences of disease-resistant genes.
【作者单位】： 山西大学生命科学学院;山西省农业科学院作物科学研究所/作物遗传与分子改良山西省重点实验室;山西省农业科学院旱地农业研究中心;北京市农林科学院杂交小麦工程技术研究中心;山西省农业科学院小麦研究所;
【基金】：国家自然科学基金项目(31171839,31401385) 山西省青年基金项目(2015021145) 山西省农业攻关项目(20150311001-1,20150311001-5) 山西省农业科学院攻关项目(15YGG01) 北京市农林科学院青年基金项目(QNJJ201428)资助~~
【分类号】：S512.1
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本文编号：1992566