中间锦鸡儿CiABR17基因的克隆及表达分析

发布时间：2018-06-09 14:04

本文选题：中间锦鸡儿 + ABR　；参考：《西北植物学报》2017年09期

【摘要】：该研究采用blastn比对方法,从中间锦鸡儿干旱胁迫抑制消减杂交文库筛选出ABA响应蛋白17基因(ABR17)的EST序列。RACE扩增得到cDNA序列594bp,其中ORF为471bp,编码157个氨基酸,预测蛋白质分子量为16.44kD,等电点为4.93,平均疏水指数为-0.126,是一种亲水蛋白。实时荧光定量PCR检测发现,中间锦鸡儿CiABR17基因在脱水、NaCl和热胁迫下均有不同程度表达,说明CiABR17基因与中间锦鸡儿响应逆境胁迫有关;过表达CiABR17拟南芥株系的鲜重、干重和莲座直径均极显著高于野生型株系。
[Abstract]:In this study, the blastn sequence 594 BP was amplified from drought stress suppression subtractive hybridization library of Caragana Intermediate Caragana by EST sequence. Race amplification of ABR17 gene, in which ORF is 471 BP, encodes 157 amino acids. The predicted molecular weight of protein is 16.44 kD, the isoelectric point is 4.93, and the average hydrophobic index is -0.126, which is a hydrophilic protein. The results of real-time fluorescence quantitative PCR showed that CiABR17 gene was expressed in different degree under dehydration NaCl and heat stress, which indicated that CiABR17 gene was related to stress response of Caragana intermedia, and the fresh weight of Arabidopsis thaliana strain CiABR17 was overexpressed. Both dry weight and rosette diameter were significantly higher than those of wild-type lines.
【作者单位】：内蒙古农业大学生命科学学院;
【基金】：国家自然科学基金(31360169) 内蒙古自治区科技创新团队(201503004)
【分类号】：Q943.2;S793.3

【相似文献】