驯鹿干扰素β1基因的克隆及遗传进化分析

发布时间：2018-06-10 10:59

本文选题：驯鹿 + IFNβ基因　；参考：《中国畜牧兽医》2017年12期

【摘要】：试验旨在从驯鹿鹿茸顶端组织中克隆干扰素β1(interferon beta 1,IFNβ1)基因全长序列,分析其分子特性,为研究其生物学活性及实际应用奠定基础。根据GenBank数据库中牛、羊等近缘物种IFNβ1基因的保守序列设计1对引物,以驯鹿鹿茸顶端间充质层组织基因组DNA为模板,采用PCR技术扩增得到驯鹿IFNβ1基因并克隆、测序,利用相关生物信息学软件对该序列进行分析。结果发现,驯鹿IFNβ1基因全长561bp,共编码186个氨基酸,含有3个糖基化位点;其编码蛋白含有4个α螺旋、4个β折叠区、7个β转角。将驯鹿IFNβ1基因推导的氨基酸序列与其他14种哺乳动物进行遗传进化分析发现,其同源性为45.1%~92.0%;驯鹿与其他动物IFNβ1基因序列分析和系统进化树分析表明,IFNβ1基因存在种属差异性,亲缘关系越近,同源性越高。本研究结果为驯鹿IFNβ1基因的进一步研究和应用提供了基础试验数据。
[Abstract]:The aim of the experiment was to clone the full length sequence of interferon 尾 1(interferon beta 1 (IFN 尾 1) gene from the apical tissue of deer antler, and to analyze its molecular characteristics, and to lay a foundation for the study of its biological activity and practical application. According to the conserved sequence of IFN- 尾 1 gene of cattle, sheep and other related species in GenBank database, a pair of primers were designed. The reindeer IFN 尾 1 gene was amplified by PCR and cloned and sequenced. The sequence was analyzed by bioinformatics software. The results showed that the total length of IFN 尾 1 gene of reindeer was 561 BP, encoding 186 amino acids and containing 3 glycosylation sites, and the coding protein contained 4 伪 helix, 4 尾 folding regions and 7 尾 turning angles. The amino acid sequence derived from the IFN 尾 1 gene of reindeer was analyzed by genetic evolution analysis with 14 other mammals, the homology was 45.1 and 92.0, and the sequence analysis of IFN 尾 1 gene and phylogenetic tree analysis of reindeer and other animals showed that the IFN- 尾 1 gene of reindeer was different in species. The closer the relationship is, the higher the homology is. The results provide basic experimental data for further study and application of IFN 尾 1 gene in reindeer.
【作者单位】：东北林业大学野生动物资源学院;
【基金】：国家林业局珍稀濒危物种野外救护与繁育项目(2012) 中央高校基本科研业务费专项资金项目(2572016AA42)
【分类号】：S825

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