马氏珠母贝磺基转移酶PmCHST11基因的分子特征与表达分析

发布时间：2018-06-11 12:08

本文选题：马氏珠母贝 + 硫酸软骨素　；参考：《基因组学与应用生物学》2017年01期

【摘要】：硫酸软骨素(CS)具有抗炎、抗病毒和软骨保护功能等免疫学功能。硫酸软骨素磺基转移酶CHST11(carbohydrate sulfotransferase 11,chondroitin 4)催化磺酸基从供体PAPS(3'-磷酸腺苷酰-5'-磷酸硫酸盐)转移到软骨素N-乙酰半乳糖胺的4位碳上,实现磺酸化反应,是合成硫酸软骨素的关键酶。本研究通过对马氏珠母贝磺基转移酶基因Pm CHST11(Pinctada martensii carbohydrate sulfotransferase 11)的全长克隆及组织表达分析,探究Pm CHST11在马氏珠母贝免疫调节中的作用。结果表明:Pm CHST11序列全长为1 418 bp,编码426个氨基酸;Pm CHST11序列含有信号肽结构区域,跨膜结构域和磺基转移酶-2结构域,为高尔基体膜偶联磺基转移酶;多序列比对结果显示,Pm CHST11与其它物种的CHST11同源性较低;实时荧光定量结果表明,Pm CHST11基因在多个组织中均有表达,其中在外套膜边缘区显著高表达(p0.05)。本研究为分析Pm CHST11在马氏珠母贝中的免疫调节中的作用积累基础资料。
[Abstract]:Chondroitin sulfate (CSS) has anti-inflammatory, anti-viral and cartilage protection functions. Chondroitin Sulfate (CHST11C) carbohydrate sulfotransferase 11chondroitin 4) catalyzes the transfer of sulfonic group from donor PAPS3 to 4 carbon of chondroitin N-acetyl galactosamine, which is the key enzyme for the synthesis of chondroitin sulfate. In this study, the full-length cloning and tissue expression analysis of Pm CHST11Pinctada martensii carbohydrate sulfotransferase 11 were carried out to explore the role of pm CHST11 in the immune regulation of Pinctada martensii. The results showed that the full length of the 1 418 BP fragment of the 1: pm CHST11 sequence, encoding 426 amino acids Pm CHST11, contained signal peptide domain, transmembrane domain and sulfonyltransferase 2 domain, and was a Golgi body membrane coupled sulfonyltransferase. The results of multiple sequence alignment showed that the homology of Pm CHST11 with other species was low, and the real-time fluorescence quantitative analysis showed that Pm CHST11 gene was expressed in many tissues, especially in the marginal region of coat membrane. This study was intended to accumulate basic data for the analysis of the role of pm CHST11 in immunomodulation of Eupatorium martensii.
【作者单位】：广东海洋大学水产学院;广东省珍珠养殖与加工工程技术研究中心;
【基金】：广东省海洋与渔业局“马氏珠母贝优质抗逆选系苗种规模化繁育与示范养殖”(Z2014009) 广东省科技厅“广东省珍珠养殖与加工工程技术研究中心建设(2016B090920096)” 广东省教育厅“马氏珠母贝高精度遗传连锁图谱构建与生长性状QTLs的精细定位”(GDOU2014050207)共同资助
【分类号】：S917.4

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